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* Corresponding Author
1H NMR Fingerprinting of Medicinal Herbs Contain Chemical Drug Material Allopurinol
https://doi.org/10.22146/mot.42300 Adita Yuniati Puspitasari(1*), Harno Dwi Pranowo(2), Respati Tri Swasono(3), Tri Rini Nuringtyas(4)
(1) Department of Chemistry, Faculty of Mathematics and Natural Science (FMIPA) Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Science (FMIPA) Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Science (FMIPA) Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Biochemistry Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
A study to differentiate the pure medicinal herbs from the mix medicinal herbs with chemical drug material has been done. For this purpose, we conducted fingerprinting of commercial medicinal herbs and chemical drug material allopurinol using 1H-NMR followed with chemometrics analysis. Nine commercial traditional herbal medicines claimed for rheumatic were used as samples as well as allopurinol as the chemical drug standard. Extraction of samples was done by ultrasonicator for 15 min in methanol-d4 containing 0.01% TMSP as an internal standard. Each type of herbal medicine was prepared in three replicates. The phytochemical analysis was done by 500 MHz JEOL NMR. The chemometrics analysis was done using SIMCA software following the 1H NMR spectra processing with MNOVA software. All spectra showed no contamination with allopurinol. The specific signals of allopurinol at aromatic regions were confirmed not present when the spectra were stacked together. Hence, the result of OPLS-DA analysis convinced that the herbs were clearly separated the medicinal herbs into 3 classes. Jamu 1 is separated from others showed very high intensity of several signals which may indicate an addition of chemical medicines but not allopurinol. The clear separation of other two groups may corresponds to the similarity of ingredients. These results also showed that most of traditional medicines which produced by small industries, the traditional medicines contain no active pharmaceutical ingredients (allopurinol) indicating a high safety of Indonesia traditional medicines.
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Ali, N. W. (2014) ‘Development and Validation of Different Chromatographic Methods for Determination of Two Hypouricemic Drugs in Their Combined Dosage Form’, Journal of Analytical & Bioanalytical Techniques, 5(6). doi: 10.4172/2155-9872.1000211.
Anonim (1999) Badan pengawas obat dan makanan. Breithaupt H, G. G. (1981) ‘Determination of allopurinol and oxipurinol in biological fluids by high-performance liquid chromatography’, J Chromatography, 226, p. 4347.
Candra, A. (2012) Waspada, Banyak Jamu Dicampur Bahan Kimia Obat! - Kompas. - Kompas.com.htm.
Chan, K. M. et al., (2010) ‘Proficiency tests for contaminants in food and herbal medicine in the Asia Pacific region’, TrAC - Trends in Analytical Chemistry. Elsevier Ltd, 29(6), pp. 562–576. doi: 10.1016/j.trac.2010.02.014.
Chen, A. et al., (2017) ‘A holistic strategy for quality and safety control of traditional Chinese medicines by the “iVarious” standard system’, Journal of Pharmaceutical Analysis. Elsevier B.V., 7(5), pp. 271–279. doi: 10.1016/j.jpha.2017.07.008.
Chen, Y. G. et al., (2014) ‘Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using1H NMR spectroscopy and multivariate statistical analysis’, Journal of Pharmaceutical and Biomedical Analysis. Elsevier B.V., 93, pp. 51–58. doi: 10.1016/j.jpba.2013.05.017.
Deborde, C. et al., (2017) ‘Plant metabolism as studied by NMR spectroscopy’, Progress in Nuclear Magnetic Resonance Spectroscopy. Elsevier B.V., 102–103, pp. 61–97. doi: 10.1016/j.pnmrs.2017.05.001.
Dona, A. C. et al., (2016) ‘A guide to the identification of metabolites in NMR-based metabonomics/metabolomics experiments’, Computational and Structural Biotechnology Journal. Natrix Separations, 14, pp. 135–153. doi: 10.1016/j.csbj.2016.02.005.
Elfahmi, Woerdenbag, H. J. and Kayser, O. (2014) ‘Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use’, Journal of Herbal Medicine. Elsevier GmbH., 4(2), pp. 51–73. doi: 10.1016/j.hermed.2014.01.002.
Harris, E. S. J. et al., (2011) ‘Heavy metal and pesticide content in commonly prescribed individual raw Chinese Herbal Medicines’, Science of the Total Environment, 409(20), pp. 4297–4305. doi: 10.1016/j.scitotenv.2011.07.032.
Heyman, H. M. and Meyer, J. J. M. (2012) ‘NMR-based metabolomics as a quality control tool for herbal products’, South African Journal of Botany. South African Association of Botanists, 82, pp. 21–32. doi: 10.1016/j.sajb.2012.04.001.
Hohmann, M. et al., (2014) ‘1H NMR profiling as an approach to differentiate conventionally and organically grown tomatoes’, Journal of Agricultural and Food Chemistry, 62(33), pp. 8530–8540. doi: 10.1021/jf502113r.
Khan, W. et al., (2017) ‘Chromatographic profiling of Pancharishta at different stages of its development using HPTLC, HPLC, GC–MS and UPLC–MS’, Phytochemistry Letters, 20(September 2016), pp. 391–400. doi: 10.1016/j.phytol.2017.04.034.
Kim, E. J. et al., (2011) ‘Metabolite profiling of angelica gigas from different geographical origins using1H NMR and UPLC-MS analyses’, Journal of Agricultural and Food Chemistry, 59(16), pp. 8806–8815. doi: 10.1021/jf2016286.
Kim, H. K., Choi, Y. H. and Verpoorte, R. (2010) ‘NMR-based metabolomic analysis of plants’, Nature Protocols, 5(3), pp. 536–549. doi: 10.1038/nprot.2009.237.
Lefort, N. et al., (2014) ‘1H NMR metabolomics analysis of the effect of dichloroacetate and allopurinol on breast cancers’, Journal of Pharmaceutical and Biomedical Analysis. Elsevier B.V., 93, pp. 77–85. doi: 10.1016/j.jpba.2013.08.017.
Malz, F. and Jancke, H. (2005) ‘Validation of quantitative NMR’, Journal of Pharmaceutical and Biomedical Analysis, 38(5 SPEC. ISS.), pp. 813–823. doi: 10.1016/j.jpba.2005.01.043.
Nourudin W Ali, Nada S Abdelwahab, H. M. E. F. and W. M. O. (2014) ‘Development and Validation of Different Chromatographic Methods for Determination of Two Hypouricemic Drugs in Their Combined Dosage Form’, Journal of Analytical & Bioanalytical Techniques, 5(6). doi: 10.4172/2155-9872.1000211.
Nuringtyas, T. R. et al., (2012) ‘Differential tissue distribution of metabolites in Jacobaea vulgaris, Jacobaea aquatica and their crosses’, Phytochemistry. Elsevier Ltd, 78, pp. 89–97. doi: 10.1016/j.phytochem.2012.03.011.
Pauli, G. F., Jaki, B. U. and Lankin, D. C. (2005) ‘Quantitative 1H NMR: Development and potential of a method for natural products analysis’, Journal of Natural Products, 68(1), pp. 133–149. doi: 10.1021/np0497301.
Preto, M. S. M. et al., (2013) ‘Determination of herb authenticity by low-field NMR’, Food Chemistry. Elsevier Ltd, 136(3–4), pp. 1272–1276. doi: 10.1016/j.foodchem.2012.09.045.
Rahmatullah, S. and Fikri, A. (2018) ‘Analisis Kualitatif Kandungan Bahan Kimia Obat (BKO) Dalam Jamu Asam Urat The 7 th University Research Colloqium 2018 STIKES PKU Muhammadiyah Surakarta’, pp. 566–575.
Refat, M. S., Mohamed, G. G. and Fathi, A. (2010) ‘Spectrophotometric determination of allopurinol drug in tablets: Spectroscopic characterization of the solid CT complexes’, Bulletin of the Korean Chemical Society, 31(6), pp. 1535–1542. doi: 10.5012/bkcs.2010.31.6.1535.
Rundlöf, T. et al., (2014) ‘Use and qualification of primary and secondary standards employed in quantitative1H NMR spectroscopy of pharmaceuticals’, Journal of Pharmaceutical and Biomedical Analysis. Elsevier B.V., 93, pp. 111–117. doi: 10.1016/j.jpba.2013.09.010.
Sen, S. and Chakraborty, R. (2017) ‘Revival, modernization and integration of Indian traditional herbal medicine in clinical practice: Importance, challenges and future’, Journal of Traditional and Complementary Medicine. Elsevier Ltd, 7(2), pp. 234–244. doi: 10.1016/j.jtcme.2016.05.006.
Shaban, N. S., Abdou, K. A. and Hassan, N. E.-H. Y. (2016) ‘Impact of toxic heavy metals and pesticide residues in herbal products’, Beni-Suef University Journal of Basic and Applied Sciences. Elsevier B.V., 5(1), pp. 102–106. doi: 10.1016/j.bjbas.2015.10.001.
Simmler, C. et al., (2014) ‘Universal quantitative NMR analysis of complex natural samples’, Current Opinion in Biotechnology, 25, pp. 51–59. doi: 10.1016/j.copbio.2013.08.004.
Tripathy, V. et al., (2015) ‘Residues and contaminants in medicinal herbs - A review’, Phytochemistry Letters. Phytochemical Society of Europe, 14, pp. 67–78. doi: 10.1016/j.phytol.2015.09.003.
Wang, L. et al., (2015) ‘Safety issues and new rapid detection methods in traditional Chinese medicinal materials’, Acta Pharmaceutica Sinica B. Elsevier, 5(1), pp. 38–46. doi: 10.1016/j.apsb.2014.12.005.
Windarsih, A. (2018) APPLICATION OF TLC AND 1H-NMR SPECTRA BASED METABOLITE FINGERPRINTING COMBINED WITH CHEMOMETRICS AS AN INTEGRATED STUDY FOR THE AUTHENTICATION OF Curcuma longa. Gadjah Mada University.
Zhang, Z. Z. et al., (2016) ‘Integrative drug efficacy assessment of Danggui and European Danggui using NMR-based metabolomics’, Journal of Pharmaceutical and Biomedical Analysis. Elsevier B.V., 120, pp. 1–9. doi: 10.1016/j.jpba.2015.12.001.
DOI: https://doi.org/10.22146/mot.42300
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Faculty of Pharmacy
Universitas Gadjah Mada