Komponen Flavor Volatil Tempe yang Dibungkus dengan Daun Pisang dan Plastik

https://doi.org/10.22146/agritech.24720

Rasyid Hanafi Harahap(1*), Zulkifli Lubis(2), Jamaran Kaban(3)

(1) Program Studi Ilmu Pangan, Fakultas Pertanian, Universitas Sumatera Utara, Jl. Prof. Dr. A. Sofyan No. 3, Kampus USU Medan Sumatera Utara 20155
(2) Program Studi Ilmu Pangan, Fakultas Pertanian, Universitas Sumatera Utara, Jl. Prof. Dr. A. Sofyan No. 3, Kampus USU Medan Sumatera Utara 20155
(3) Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA), Universitas Sumatera Utara, Jl. Bioteknologi No. 1 Kampus USU Medan Sumatera Utara 20155
(*) Corresponding Author

Abstract


The objective of the research was to identify volatile flavor compounds and odor description of tempeh wrapped with banana leaf and plastic fermented at different times. The research object was tempeh wrapped with banana leaf (TD) at 48 (TD1H), 72 (TD2H), and 96 hours (TD3H) fermented, and tempeh wrapped with plastic (TP) at 48 (TP1H), 72 (TP2H), and 96 hours (TP3H) fermented. Sample was extracted by SPME. GC-MS/O was used to identify volatile flavor compounds and odor description. The volatile flavor compound found in tempeh were group of ester, terpenoid, alcohol, aldehyde, ketone, furan and nitrogen containing compound. α-pinene was only found in tempeh wrapped with banana leaf, whereas piperazine, sec-butyl nitrite and (Z)-α-bisabolene were only found in tempeh wrapped with plastic. This revealed that there was difference flavor compound found in tempeh wrapped with banana leaf and tempeh wrapped with plastic.

 

ABSTRAK

Penelitian ini bertujuan untuk mengetahui komponen penyusun flavor volatil dan aroma tempe yang dibungkus daun pisang dan plastik pada waktu fermentasi yang berbeda-beda. Tempe yang digunakan sebagai objek penelitian adalah tempe yang dibungkus dengan daun pisang (TD) dengan waktu fermentasi 48 jam (TD1H), 72 jam (TD2H), dan 96 jam (TD3H) dan tempe yang dibungkus plastik (TP) dengan waktu fermentasi 48 jam (TP1H), 72 jam (TP2H), dan 96 jam (TP3H). Ekstraksi sampel tempe menggunakan HS-SPME. Analisis flavor dan deskripsi odor dilakukan dengan menggunakan GC-MS/O. Senyawa flavor tempe yang diperoleh adalah senyawa-senyawa dari golongan ester, terpenoid, alkohol, aldehid, keton, furan dan senyawa-senyawa yang mengandung nitrogen. α-pinen hanya ditemukan pada tempe yang dibungkus daun pisang, sedangkan piperazin, sec-butil nitrit dan (Z)-α-bisabolen hanya ditemukan pada tempe yang dibungkus plastik saja. Hal ini berarti ada perbedaan komponen penyusun senyawa flavor pada tempe yang dibungkus daun pisang dan plastik.


Keywords


Flavor; GC-MS/O; SPME; tempeh

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References

Apriyantono, A., Nurkori, Nurjanah, S., & Satiawihardja, B. (2001). Food Flavors and Chemistry. In A. M. Spanier, F. Shahidi, T. H. Parliment, C. Mussinan, C.-T. Ho, & E. T. Contis (Eds.), Food Flavors and Chemistry Advances of the New Millennium (pp. 171–182). Cambridge: The Royal Society of Chemistry.

Babushok, V. I., Linstrom, P. J., & Zenkevich, I. G. (2011). Retention Indices for Frequently Reported Compounds of Plant Essential Oils. Journal of Physical and Chemical Reference Data, 40(4). https://doi.org/10.1063/1.3653552

Bianchi, F., Careri, M., Mangia, A., & Musci, M. (2007). Retention indices in the analysis of food aroma volatile compounds in temperature-programmed gas chromatography: Database creation and evaluation of precision and robustness. Journal of Separation Science, 30(4), 563–572. https://doi.org/10.1002/jssc.200600393

Buttery, R. G., Orts, W. J., Takeoka, G. R., & Nam, Y. (1999). Volatile flavor components of rice cakes. Journal of Agricultural and Food Chemistry, 47(10), 4353–4356. https://doi.org/10.1021/jf990140w

Carballo, J. (2012). The Role of Fermentation Reaction in the Generation of Flavor and Aroma of foods. In B. M. Mehta, A. Kamal-Eldin, & R. Z. Iwanski (Eds.), Book Fermentation Effects on Food Properties (pp. 51–88). New York: CRC Press.

Choi, H.-S. (2003). Character impact odorants of Citrus hallabong (Citrus unshiu Marcov x Citrus sinensis Osbeck) x C. reticulata Blanco) cold pressed peel oil. Journal of Agricultural and Food Chemistry, 51(9), 2687–2692. https://doi.org/10.1021/jf021069o

Elmore, J. S., Campo, M. M., Enser, M., & Mottram, D. S. (2002). Effect of lipid composition on meat-like model systems containing cysteine, ribose, and polyunsaturated fatty acids. Journal of Agricultural and Food Chemistry, 50(5), 1126–1132. https://doi.org/10.1021/jf0108718

Fan, W., & Qian, M. C. (2005). Headspace solid phase microextraction and gas chromatography-olfactometry dilution analysis of young and aged Chinese “Yanghe Daqu” liquors. Journal of Agricultural and Food Chemistry, 53(20), 7931–7938. https://doi.org/10.1021/jf051011k

Goodner, K. L. (2008). Practical retention index models of OV-101, DB-1, DB-5, and DB-Wax for flavor and fragrance compounds. LWT - Food Science and Technology, 41(6), 951–958. https://doi.org/10.1016/j.lwt.2007.07.007

Högnadóttir, Á., & Rouseff, R. L. (2003). Identification of aroma active compounds in orange essence oil using gas chromatography-olfactometry and gas chromatography-mass spectrometry. Journal of Chromatography A, 998(1–2), 201–211. https://doi.org/10.1016/S0021-9673(03)00524-7

Jeleń, H., Majcher, M., Ginja, A., & Kuligowski, M. (2013). Determination of compounds responsible for tempeh aroma. Food Chemistry, 141(1), 459–465. https://doi.org/10.1016/j.foodchem.2013.03.047

Kustyawati, M. E. (2009). Kajian peran yeast dalam pembuatan tempe. Agritech, 29(2), 64–70.

Liu, Y., Miao, Z., Guan, W., & Sun, B. (2012). Analysis of organic volatile flavor compounds in fermented stinky tofu using SPME with different fiber coatings. Molecules, 17(4), 3708–3722. https://doi.org/10.3390/molecules17043708

Mei Feng, X., Ostenfeld Larsen, T., & Schnürer, J. (2007). Production of volatile compounds by Rhizopus oligosporus during soybean and barley tempeh fermentation. International Journal of Food Microbiology, 113(2), 133–141. https://doi.org/10.1016/j.ijfoodmicro.2006.06.025

Rouseff, R. L., Onagbola, E. O., Smoot, J. M., & Stelinski, L. L. (2008). Sulfur volatiles in guava (Psidium guajava L.) leaves: Possible defense mechanism. Journal of Agricultural and Food Chemistry, 56(19), 8905–8910. https://doi.org/10.1021/jf801735v

Shurtleff, W., & Aoyagi, A. (1979). The Book of Tempeh. New York: Harper and Row Publishers.

Supriyanto, Wada, K., Hayakawa, I., & Fujio, Y. (1991). Principal Component Analysis of Aroma Profiles of Tempeh Produced by Using Various Starters. Hakkokogaku, 69, 331–336.

Van Ba, H., Ryu, K. S., & Inho, H. (2012). Flavor characteristics of Hanwoo beef in comparison with other Korean foods. Asian-Australasian Journal of Animal Sciences, 25(3), 435–446. https://doi.org/10.5713/ajas.2011.11286

Vranová, J., & Ciesarová, Z. (2009). Furan in food - A review. Czech Journal of Food Sciences.

Yaakob, H., Malek, R. A., Misson, M., Jalil, M. F. A., Sarmidi, M. R., & Aziz, R. (2011). Optimization of isoflavone production from fermented soybean using response surface methodology. Food Science and Biotechnology, 20(6), 1525–1531. https://doi.org/10.1007/s10068-011-0211-6

Yaylayan, V. A. (2006). Precursors, formation and determination of furan in food. Journal Fur Verbraucherschutz Und Lebensmittelsicherheit, 1(1), 5–9. https://doi.org/10.1007/s00003-006-0003-8



DOI: https://doi.org/10.22146/agritech.24720

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