Current Topics in Molecular Plant Pathology

https://doi.org/10.22146/jpti.10068

Keiko T. Natsuaki(1*)

(1) Department of International Agricultural Development Tokyo University of Agriculture (TUA)
(*) Corresponding Author

Abstract


Some current topics on molecular technology in plant pathology are reviewed. For detection and identification of plant pathogens, PCR with various modifications is applied. Study of nucleic acids sequence of pathogens gives keys for classification. For disease management, investigation of host reaction is also significant and mechanism of resistant genes is studied. In the study of plant reaction against pathogens, gene silencing is focused and compared with programmed cell death of animals. Since cost of molecular technology is still expensive, plant pathologists in Asia are expected to utilize their conventional techniques to realize ecologically sound plant protection for sustainable crop production.


Keywords


PCR; resistant genes; gene silencing; plant protection

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References

Gebhardt, C. & J.P. Valkonen. 2001. Organization of Genes Controlling Disease Resistance in the Potato Genome. Annu. Rev. Phytopathol. 39: 79─102.

Dinesh-Kumar, S.P. & B. Baker. 2000. Alternatively Spliced N Resistance Gene Transcripts: Their Possible Role in Tobacco Mosaic Virus Resistance. Proc. Nati. Acad. Sci. USA 97: 1908─1913.

Singh, R.P. & U.S. Singh. 1995. Molecular Methods in Plant Pathology. CRC Lewis Publisher. Boca Raton FL. 560p.

Zeigler, R.S. 2001. Agricultural Biotechonology, Reducing Poverty in Developing Countries. Plant Disease 85: 568─579.

Sharman, M., J.E. Thomas & R.G. Dietzgen. 2000. Development of a Multiplex Immunocapture PCR with Colorimetric Detection for Viruses of Banana. J. Virol. Methods 89: 75─88.

Martin, G.B., A. Frary, T. Wu, S. Brommonschenkel, J. Chunwongse, E.D. Earle & S.D. Tanksley. 1994. A Member of the Tomato Pto Gene Family Confers Sensitivity to Fenthion Resulting in Rapid Cell Death. Plant Cell 6: 1543─1552.

Whitham, S., S.P. Dinesh-Kumar, D. Choi, R. Hehl, C. Corr, & B. Baker. 1994. The Product of the Tobacco Mosaic Virus Resistance Gene N: Similarity to Toll and the Interleukin-1Receptor. Cell 78: 1101─1115.

Schena, M., D. Shalon, R. Davis & P.O.Brown. 1995. Quantitative Monitoring of Gene Expression Patterns with a cDNA Microarray. Science 270: 467─470.

Brown, P.O. & D. Botstein. 1999. Exploring the New World of the Genome with DNA Microarrays. Nature Genetics 21: 33─37.

Martin, G., M. Ganal & S.D. Tanksley. 1992. Construction of a Yeast Artificial Chromosome Library of Tomato and Identification of Cloned Segments Linked to Two Disease Resistance Loci. Mol. Gen. Genetics 233: 25─3 2.

Hamilton, A.J. & D.C. Baulcombe. 1999. A Novel Species of Small Antisense RNA in Post-transcriptional Gene Silencing. Science 286: 950─952.



DOI: https://doi.org/10.22146/jpti.10068

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