* Corresponding Author
Virulensi Fusarium oxysporum f.sp. zingiberi Isolat Boyolali dan Temanggung setelah Disimpan Enam Tahun dalam Tanah Steril
https://doi.org/10.22146/jpti.11891 Andry Slamet Riyadi(1*), Loekas Soesanto(2), Kustantinah Kustantinah(3)
(1) Fakultas Pertanian Universitas Jenderal Soedirman
(2) Fakultas Pertanian Universitas Jenderal Soedirman
(3) Fakultas Pertanian Universitas Jenderal Soedirman
(*) Corresponding Author
Abstract
This research aimed to study growth ability and virulence of several Fusarium oxysporum f.sp. zingiberi isolates originated from Boyolali and Temanggung after being preserved for six years in sterile soils media. Completely Randomized Design was used with 12 treatments and 3 replications. F. oxysporum f.sp. zingiberi originated from Temanggung consisted of 7 isolates, i.e., TKO1, TKO3, TKO4, TKO6, TKO7, TPO1, TPO5; and from Boyolali consisted of 4 isolates, i.e., BAO2, BAO7, BAC, and BAP. Variables observed were growth on PDA, colony color, colony diameter, macroconidia and microconidia, mycelial dry weight, incubation period, attack area, and difference of fresh weight of rhizome. The result showed that all isolates of F. oxysporum f.sp. zingiberi both from Temanggung and Boyolali were able to grow well on PDA and fully covered the Petridish at 4.75–7.5 days. The most virulent isolate was TKO6 from Temanggung showing the fastest incubation period of 5 days after inoculation and the highest attack area of 420 mm2 or increase for 107.6%.
Keywords
Full Text:
PDFReferences
Agrios, G.N. 2005. Plant Pathology, 5th edition. Academic Press, New York. 922 p.
Ahn, I.P. & Y.H. Lee. 2000. Vegetative Compatibility Groups and Pathogenicity Variation among Isolates Fusarium oxysporum f.sp. melonis. Plant Pathology Journal 16:227–230.
Amalia, R., H.A. Djatmiko, & L. Soesanto 2004. Potensi Beberapa Antagonis dalam Menekan Fusarium oxysporum Schlecht f.sp. zingiberi Trujillo pada Tanaman Jahe. p. 301–312. In L. Soesanto (ed.), Prosiding Simposium Nasional I tentang Fusarium, Purwokerto 26–27 Agustus 2004.
Arifah, N. 2004. Kisaran Inang Fusarium oxysporum Schlecht. f.sp. zingiberi Trujillo in Planta. Skripsi. Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto. (Tidak dipublikasikan).
Atkinson, R.G. 1954. Quantitative Studies on the Survival of Fungi in Five-year Old Dried Soil Cultures. Canadian Journal of Botany 32: 673–678.
Dahmen, H., Th. Staub, & F.J. Schwinn. 1983. Technique for Longterm Preservation of Phytopathogenic Fungi in Liquid Nitrogen. Phytopathology 73: 241–246.
Diogo, H.C.,A. Sarpieri, &M.C. Pires. 2005. Fungi Preservation in DistilledWater. Anais Brasileiros de Dermatologia 80: 591–594.
Domsch, K.H., W. Gams, & T-H. Anderson. 1993. Compendium of Soil Fungi. IHW-Verlag, Eching. 859 p.
Elmer,W.H. 1991. Vegetative Compatibility Groups of Fusarium proliferatum from Asparagus and Comparisons of Virulence, Growth Rate, and Colonization of Asparagus Residues among Groups. Phytopathology 81: 852–857.
Jullien,M. 1988. Effect of the Fusarium spp. Toxins and Selection of Crude Toxin Resistant Strains in Mesophyll Cell Cultures of Asparagus officinalis. Plant Physiology & Biochemistry 26: 713–722.
Kistler, H.C. 1997. Genetic Diversity in the Plant-pathogenic Fungus Fusarium oxysporum. Pythopathology 87: 474–479.
Leslie, J.F., B.A. Summerell, & S. Bullock. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Professional, Iowa. 385 p.
Megnegneau, B. &M. Branchard. 1988. Toxicity of Fusaric Acid Observed on Callus of Various Cucumis melo Genotypes. Plant Physiology and Biochemistry 26: 585–588.
Nagai, T., A. Ideno, M. Tsuge, C. Oyanagi, M. Oniki, K. Kita,M. Horita, T.Aoki, T. Kobayashi, & K. Touchiya. 2000. Preservation of Fungi in an Atmosphere Over Liquid Nitrogen after Uncontrolled Freezing. Microbiology Culture Collection 16: 13–22.
Nakasone, K.K., S.W. Peterson, & S.C. Jong. 2004. Preservation and Distribution of Fungal Cultures. p. 37–47. In G.M. Mueller, G.F. Bills, & M.S. Foster (eds.), Biodiversity of Fungi, Inventory and Monitoring Methods. Elsevier Academic Press, Amsterdam.
Pancasiwi, D. 2004. Uji Ketahanan Beberapa Varietas Jahe terhadap F. oxysporum f.sp. zingiberi Secara In Vitro dan In Planta. Skripsi. Purwokerto: Universitas Jenderal Soedirman.
Pasarell, L. & M.R. McGinnis. 1992. Viability of Fungal Cultures Maintained at -70ºC. Journal of Clinical Microbiology 30: 1000–1004.
Perez-Garcia, A., E. Mingorance, M.E. Rivera, D. del Poso, D. Romero, J.A. Tores, & A. de Vicente. 2006. Longterm Preservation of Podosphaera fusca Using Silica Gel. Journal of Phytopathology 154: 190–192.
Prabowo, A.K.E., N. Prihatiningsih, & L. Soesanto. 2006. Potensi Trichoderma harzianum dalam Mengendalikan Sembilan Isolat Fusarium oxysporum Schlecht. f.sp. zingiberi Trujillo pada Kencur. Jurnal Ilmu-Ilmu Pertanian Indonesia 8: 76–84.
Semangun, H. 2000. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Gadjah Mada University Press. Yogyakarta. 850 p.
Soesanto, L., Soedharmono, N. Prihatiningsih, A. Manan, E. Iriani, & J. Pramono. 2003. Penyakit Busuk Rimpang Jahe di Sentra Produksi Jahe Jawa Tengah: 1. Identifikasi dan Sebaran. Tropika 11: 178–185.
Soesanto, L., Y.P. Dewi, & N. Prihatiningsih. 2005. Pengenalan Dini Penyakit Busuk Rimpang Jahe. Jurnal Penelitian Pertanian Agrin 8: 76–83.
Suciatmih & Rachmat. 2005. Pengujian Survival Jamur yang Dipreservasi dalam Air dan Parafin Cair. Berita Biologi 7: 241–248.
Wahyu, H.S.N. 2008. Uji Keagresifan Fusarium oxysporum f.sp. zingiberi Beberapa Isolat Temanggung dan Boyolali pada Jahe Gajah setelah Disimpan Tiga Tahun. Skripsi. Purwokerto: Universitas Jenderal Soedirman. Purwokerto.
Winarni, W., E. Pramono, Soedarmono, & L. Soesanto. 2004. Uji Kepatogenan Beberapa Isolat Fusarium oxysporum f.sp. zingiberi pada Tanaman Jahe Gajah. p. 128–136. In L. Soesanto (ed.), Prosiding Simposium Nasional I tentang Fusarium, Purwokerto 26–27 Agustus 2004.
Windels, C.E., P.M. Burnes, & T. Kommendahl. 1988. Five-year Preservation of Fusarium Species on Silica Gels and Soil. Phytopathology 78:107–109.
DOI: https://doi.org/10.22146/jpti.11891
Article Metrics
Abstract views : 1648
| 
views : 1861
Refbacks
- There are currently no refbacks.
Copyright (c) 2008 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics