Ekstraksi DNA Phytophthora palmivora Isolat Kelapa

https://doi.org/10.22146/jpti.9358

Achmadi Priyatmojo(1*), Siti Subandiyah(2)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium.

The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.

Key words: DNA, Phytophthora palmivora


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DOI: https://doi.org/10.22146/jpti.9358

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