Production of Polyhvdroxyalkanoates from Synthetic Wastewater Using Sequencing Batch Reactors in On-Off Sequences

Tjandra Setiadi(1*), Herriyanto Ronny Sondjaja(2), Bambang Veriansyah(3)

(1) Department of Chemical Engineering Institut Teknologi Bandung JI. GanesaNo. 10, Bandung, INDONESIA
(2) Department of Chemical Engineering Institut Teknologi Bandung JI. GanesaNo. 10, Bandung, INDONESIA
(3) Department of Chemical Engineering Institut Teknologi Bandung JI. GanesaNo. 10, Bandung, INDONESIA
(*) Corresponding Author


The biosynthesis of polyhydroxyalkanoates (PHAs) using activated sludge as cultureorganism from tapioca synthetic wastewater has been studied in a 6L sequencing batch reactor (SBR). It was found that longer aeration time caused greater chemical oxygen demand (COD) reduction but did not achieve the maximum PHA-production rate. The application of on-off sequences, however, gave an acceptable PHA- productivity rate at 0.1033 gPHA L-1 h-1 and a high storage yield of up to 0.32 gPHNg biomass, although it caused a decrease in pH and affected sludge stability. The on-off variation also produced a higher valerate copolymer content, in the range of 16.44-22.48 % for Run 1 and of 13.09-14.09 % for Run 2, compared to the customary aerated-unaerated variations. The study indicates that the use of on-off SBR sequences may be suitable for obtaining high PHA-storage yields.


Activated sludge, aerated-unaerated SBR period, polyhydroxyalkanoates (PHAs), sequencing batch reactor (SBR), and synthetic wastewater.

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ASEAN Journal of Chemical Engineering  (print ISSN 1655-4418; online ISSN 2655-5409) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada.