Microparticles Formation of Ganoderma lucidum Extract by Electrospraying Method

https://doi.org/10.22146/ajche.52004

Siti Machmudah(1*), Dwi Setyorini(2), Sugeng Winardi(3), Wahyudiono Wahyudiono(4), Hideki Kanda(5)

(1) Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
(2) Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
(3) Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
(4) Department of Materials Process Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
(5) Department of Materials Process Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
(*) Corresponding Author

Abstract


In this work, Ganoderma lucidum (G. lucidum) extract was produced in microparticles form by electrospraying. G. lucidum was extracted hydrothermally at temperature of 160oC and pressure of 7 MPa. The extract solution was subsequently mixed with 6% of Polyvinyl pyrrolidone (PVP) and formed into microparticles by electrospraying process. The electrospraying was carried out at applied voltage of 12, 14, and 16 kV, and the distance between syringe tip and electrospun collector of 8, 10, and 12 cm. The microparticles formed was analyzed using scanning electron microscope (SEM), fourier-transform infrared (FTIR) spectroscopy, and UV-Vis spectrofotometer. The antioxidant efficiency of particles was also analyzed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Based on the SEM analysis, the G. lucidum extract (GLE) – PVP spherical particles were formed by electrospraying. The finer fibres were clearly formed with the increasing applied voltage. The results showed that applied voltage and distance of tip to electrospun collector significantly influence the antioxidant efficiency and the diameter size of particles. The antioxidant efficiency increased with the rising applied voltage and gap of tip to electrospun collector, while the particle diameter decreased with the rising applied voltage and gap of tip to electrospun collector due to fast mass transfer and evaporation. The largest antioxidant efficiency of particles was 0.377/min obtained at 16 kV and 12 cm. It indicated that electrospraying is an effective process to produce pharmaceutical compounds in powder form.


Keywords


Ganoderma lucidum, electrospraying, microparticles, hydrothermal extraction

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References

  1. Akbar, R., Yam, W. K. (2011). “Interaction of ganoderic acid on HIV related target: molecular docking studies,” Bioinformation, 7, 413-417.
  2. Bishop, K. S., Kao, C. H. J., Xu, Y., Glucina, M. P., Paterson, R. R. M., Ferguson, L. R. (2015). From 2000 years of Ganoderma lucidum to recent developments in nutraceuticals,” Phytochemistry, 114, 56–65.
  3. Cai, Q., Li, Y., Pei, G. (2017). “Polysaccharides from Ganoderma lucidum attenuate microglia-mediated neuroinflammation and modulate microglial phagocytosis and behavioural response,” J. Neuroinflammation 14, 63-70.
  4. Chen, S., Yong, T., Zhang, Y., Su, J., Jiao, C., Xie, Y. (2017). “Anti-tumor and anti-angiogenic ergosterols from Ganoderma lucidum,” Front. Chem., 5, 85-70.
  5. Chhouk, K., Wahyudiono, Kanda, H., Goto, M. (2018). “Micronization for Enhancement of Curcumin Dissolution via Electrospraying Technique,” ChemEngineering, 2, 60-66.
  6. Elhassaneen, Y. A., Ragab, S. S., Salman, M. S. (2016). “The Potential Effects of Reishi Mushroom (Ganoderma lucidum) Consumption on Bone Health Indices and Serum Minerals Profile Disorders Induced by CCl4 on Rats,” Pyrex J. Medicinal Pant Res., 2, 001-007.
  7. Gaona-Sanchez, V. A., Calderon-Dominguez, G., Morales-Sanchez, E., Chanona-Perez, J. J., Arzate-Vazquez, I., Terres-Rojas, E. (2016). “Pectin-based films produced by electrospraying,” J. Appl. Polym. Sci., https://doi.org/10.1002/app.43779.
  8. Gill, B. S. and Kumar, S. (2016) “Evaluating anti-oxidant potential of ganoderic acid A in STAT 3 pathway in prostate cancer,” Mol. Biol. Rep., 43, 1411–1422.
  9. Gomez-Estaca, J., Balaguer, M. P., Gavara, R., Hernandez-Muooz, P. (2012). “Formation of zein nanoparticles by electrohydrodynamic atomization: Effect of the main processing variables and suitability for encapsulating the food coloring and active ingredient curcumin,” Food Hydrocoll., 28, 82–91.
  10. Ishimoto, Y., Ishibashi, K. -I., Yamanaka, D., Adachi, Y., Ito, H., Igami, K., Miyazaki, T., Ohno, N. (2017). “Enhanced release of immunostimulating ß-1, 3-Glucan by autodigestion of the lingzhi medicinal mushroom, ganoderma lingzhi (Agaricomycetes),” Int. J. Med. Mushrooms, 19, 1-16.
  11. Ji, Z., Tang, Q., Zhang, J., Yang, Y., Jia, W., Pan, Y. (2007). “Immunomodulation of RAW264. 7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum,” J. Ethnopharmacol., 112, 445–450.
  12. Jin, X., Beguerie, J. R., Sze, D. M.-Y., Chan, G. (2012). “Ganoderma lucidum (Reishi mushroom) for cancer treatment,” Cochrane Database Syst. Rev., 6.
  13. Krishna, K. V., Karuppuraj, V., Perumal, K. (2016). “Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum,” Asian J. Pharm. Anal., 6, 213–215.
  14. Pan, D., Zhang, D., Wu, J., Chen, C., Xu, Z., Yang, H., Zhou, P. (2014). “A novel proteoglycan from Ganoderma lucidum fruiting bodies protects kidney function and ameliorates diabetic nephropathy via its antioxidant activity in C57BL/6 db/db mice,” Food Chem. Toxicol., 63, 111–118.
  15. Pan, X., Lopez-Olivo, M. A., Song, J., Pratt, G., Suarez-Almazor, M. E. (2017). “Systematic review of the methodological quality of controlled trials evaluating Chinese herbal medicine in patients with rheumatoid arthritis,” BMJ Open, 7, e013242. doi: 10.1136/bmjopen-2016-013242.
  16. Paximada, P., Echegoyen, Y., Koutinas, A. A., Mandala, L. G., Lagaron, J. M. (2017). “Encapsulation of hydrophilic and lipophilized catechin into nanoparticles through emulsion electrospraying,” Food Hydrocoll., 64, 123–132.
  17. Rajasekaran, M. and Kalaimagal, C. (2012). “Cardioprotective effect of a medicinal mushroom, Ganoderma lucidum against adriamycin induced toxicity,” Int. J. Pharmacol., 8, 252–258.
  18. Sudheesh, N. P., Ajith, T. A., Ramnath V., Janardhanan K. K. (2010). “Therapeutic potential of Ganoderma lucidum (Fr.) P. Karst. against the declined antioxidant status in the mitochondria of post-mitotic tissues of aged mice.,” Clin Nutr., 29, 406-412.  
  19. Sliva, D. (2003) “Ganoderma lucidum (Reishi) in cancer treatment,” Integr. Cancer Ther., 2, 358–364.
  20. Wang, Y., Zhang, L., Li, Y., Hou, X., Zeng, F. (2004). “Correlation of structure to antitumor activities of five derivatives of a ß-glucan from Poria cocos sclerotium,” Carbohydr. Res., 339, 2567–2574.
  21. Yao, Z. -C., Jin, L. -J., Ahmad, Z., Huang, J., Chang, M. -W., Li, J. -S. (2017). “Ganoderma lucidum polysaccharide loaded sodium alginate micro-particles prepared via electrospraying in controlled deposition environments,” Int. J. Pharmaceutics, 524, 148–158.
  22. Zhao, D., Li, J. -S., Suen, W., Chang, M.-W., Huang, J. (2016). “Preparation and characterization of Ganoderma lucidum spores-loaded alginate microspheres by electrospraying,” Mat. Sci. Eng. C, 62, 835–842.
  23. Zhu, L. -F., Chen, X., Ahmad, Z., Li, J. -S., Chang, M. -W., (2019). “Engineering of Ganoderma lucidum polysaccharide loaded polyvinyl alcohol nanofibers for biopharmaceutical delivery,” J. Drug Deliv. Sci. Technol., 50, 2.

 



DOI: https://doi.org/10.22146/ajche.52004

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