Comparison of Maseration and Sonication Method on Flavonoid Extraction from Mango Leaves: Effect of Solvent Ratio

Alifiana Permata Sari(1), Nur Layli Amanah(2), Awalia Wardatullathifah(3), Agung Nugroho(4*)

(1) Department of Chemical Engineering, Universitas Pertamina Jalan Teuku Nyak Arief, Simprug, Kebayoran Lama, Jakarta 12220, Indonesia
(2) Department of Materials Science and Engineering, National Taiwan University of Science and Technology, No.43, Sec. 4, Keelung Road, Taipei 10607, Taiwan
(3) Department of Chemical Engineering, Universitas Pertamina Jalan Teuku Nyak Arief, Simprug, Kebayoran Lama, Jakarta 12220, Indonesia
(4) Department of Chemical Engineering, Universitas Pertamina Jalan Teuku Nyak Arief, Simprug, Kebayoran Lama, Jakarta 12220, Indonesia
(*) Corresponding Author


Mango leaf extract has proven to contain flavonoids that serve as antioxidants. In this study, a comparison between traditional maceration and sonication on flavonoid extraction from mango leaf was investigated. The various ratios of ethanol and acetone were utilized as solvents (1:5, 1:10, and 1:15). The sonication process, which uses an ultrasonic cleaning bath set at 40 oC, takes 30 minutes as contrasted to the maceration procedure of 36 hours treatment at room temperature. The flavonoid test using aluminum (III) chloride (AlCl3) colorimetric technique shows that acetone provides greater solvent power than ethanol. According to this study, the optimal ratios for the maceration and sonication procedures are 1:10 and 1:15, respectively. The maceration process resulted in the optimum extract of 0.186 mgQE/g dry leaves. Meanwhile, using a 1:15 acetone solvent ratio and the sonication method, the highest concentration of flavonoid components was discovered, reaching 0.143 mgQE/g dry material with 54 times shorter time.


Extraction, Flavonoid, Mango Leaves, Optimum Ratio, Solvent

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Al-Khayri, J.M., Sahana, G.R., Nagella, P., Joseph, B. V, Alessa, F.M., and Al-Mssallem, M.Q., 2022. “Flavonoids as potential anti-inflammatory molecules: A review.” Molecules, 27 (9), 2901

Calado, J.C.P., Albertão, P.A., de Oliveira, E.A., Letra, M.H.S., Sawaya, A.C.H.F., and Marcucci, M.C., 2015. “Flavonoid contents and antioxidant activity in fruit, vegetables and other types of food.” Agric. Sci., 6, 426.

Chaves, J.O., de Souza, M.C., da Silva, L.C., Lachos-Perez, D., Torres-Mayanga, P.C., Machado, A.P. da F., Forster-Carneiro, T., Vázquez-Espinosa, M., González-de-Peredo, A.V., Barbero, G.F., and Rostagno, M.A., 2020. “Extraction of flavonoids from natural sources using modern techniques.” Front. Chem., 8. 507887. fchem.2020.507887

Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., and Abert-Vian, M., 2017. “Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review.” Ultrason. Sonochem., 34, 540–560.

Chen, Q., Wang, X., Yuan, X., Shi, J., Zhang, C., Yan, N., and Jing, C., 2021. “Comparison of phenolic and flavonoid compound profiles and antioxidant and α-glucosidase inhibition properties of cultivated soybean (glycine max) and wild soybean (glycine soja).” Plants, 10(4), 813. plants10040813

Cornard, J.P., and Merlin, J.C., 2002. “Spectroscopic and structural study of complexes of quercetin with Al(III).” J. Inorg. Biochem., 92, 19–27.

Cruz, L., Basílio, N., Mateus, N., de Freitas, V., and Pina, F., 2022. “Natural and synthetic flavylium-based dyes: the chemistry behind the color.” Chem. Rev., 122, 1416–1481.

Dall’Acqua, S., Miolo, G., Innocenti, G., and Caffieri, S., 2012. “The photodegradation of quercetin: Relation to oxidation.” Molecules, 17(8), 8898-8907.

Dorta, E., Lobo, M.G., and Gonzalez, M., 2012. “Reutilization of mango byproducts: study of the effect of extraction solvent and temperature on their antioxidant properties.” J. Food Sci. 77, C80-8.

Gharibi, S., Tabatabaei, B.E.S., and Saeidi, G., 2015. “Comparison of essential oil composition, flavonoid content and antioxidant activity in eight achillea species.” J. Essent. Oil Bear. Plants, 18, 1382–1394.

Hamidu, L., Ahmad, A.R., and Najib, A., 2018. “Qualitative and quantitative test of total flavonoid buni fruit (Antidesma bunius (L.) Spreng) with UV-Vis spectrophotometry method.” Pharmacogn. J. 10 (1), 60-63

Hapsari, S., Yohed, I., Kristianita, R.A., Jadid, N., Aparamarta, H.W., and Gunawan, S., 2022. “Phenolic and flavonoid compounds extraction from Calophyllum inophyllum leaves.” Arab. J. Chem. 15 (3), 103666.

Jovanović, A.A., Đorđević, V.B., Zdunić, G.M., Pljevljakušić, D.S., Šavikin, K.P., Gođevac, D.M., and Bugarski, B.M., 2017. “Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques.” Sep. Purif. Technol., 179, 369–380.

Kalita, P., Tapan, B., Pal, T., and Kalita, R., 2013. “Estimation of total flavonoids content (TFC) and anti oxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn.” J. Drug Deliv. Ther., 3(4), 33-37.

Martín, M.Á., and Ramos, S., 2021. “Dietary flavonoids and insulin signaling in diabetes and obesity.” Cells, 10 (6), 1474.

Masturi, Alighiri, D., Edie, S.S., Drastisianti, A., Khasanah, U., Tanti, K.A., Susilawati, Maghfiroh, R.Z., Kirana, K.G.C., and Choirunnisa, F., 2020. “Identification of flavonoid compounds and total flavonoid content from biowaste of local durian shell (Durio zibethinus).” J. Phys. Conf. Ser., 1567, 42084.

Masturi, Alighiri, D., Nuzulina, K., Rodhiyah, M., and Drastisianti, A., 2019. “Optimization of condition extraction in quantification of total flavonoid content in the seeds of the Arummanis (Mangifera indica L.) mango from Indonesia.” J. Phys. Conf. Ser., 1321, 22041.

Maungchanburee, S., Phongseeput, S., Thongsri, O., Maijuy, M., and Chaithada, P., 2020. “Study of antioxidant activities, total phenolic content and total flavonoid content of the extracts of Monochoria vaginalis and Cissus repens Lamk. using different solvents.” J. Pharm. Sci. Res,. 12, 356–359.

Morata, A., González, C., Tesfaye, W., Loira, I., and Suárez-Lepe, J.A., 2019. “Chapter 3 - Maceration and Fermentation: New Technologies to Increase Extraction,” in: Morata, A.B.T.-R.W.T. (Ed.), . Academic Press, pp. 35–49.

Nguyen, M.P., 2020. “Efficacy of some variables of extraction to the total phenolic and flavonoid content in young mango (Mangifera indica L.) leaf.” Ann. PHYTOMEDICINE-AN Int. J., 9, 113–115.

Phuyal, N., Jha, P.K., Raturi, P.P., and Rajbhandary, S., 2020. “Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC.” Sci. World J., 2020, 8780704. 10.1155/2020/8780704

Pisoschi, A.M., Pop, A., Iordache, F., Stanca, L., Predoi, G., and Serban, A.I., 2021. “Oxidative stress mitigation by antioxidants - An overview on their chemistry and influences on health status.” Eur. J. Med. Chem., 209, 112891.

Safdar, M.N., Kausar, T., and Nadeem, M., 2017. “Comparison of ultrasound and maceration techniques for the extraction of polyphenols from the mango peel.” J. Food Process. Preserv., 41, e13028.

Sasadara, M.M. V, and Wirawan, I.G.P., 2021. “Effect of extraction solvent on total phenolic content, total flavonoid content, and antioxidant activity of Bulung Sangu (Gracilaria sp.) Seaweed.” IOP Conf. Ser. Earth Environ. Sci., 712, 12005.

Shen, J., Zou, Z., Zhang, X., Zhou, L., Wang, Y., Fang, W., and Zhu, X., 2018. “Metabolic analyses reveal different mechanisms of leaf color change in two purple-leaf tea plant (Camellia sinensis L.) cultivars.” Hortic. Res., 5, 7. 10.1038/s41438-017-0010-1

Shraim, A.M., Ahmed, T.A., Rahman, M.M., and Hijji, Y.M., 2021. “Determination of total flavonoid content by aluminum chloride assay: A critical evaluation.” LWT, 150, 111932. 10.1016/j.lwt.2021.111932

Singla, M., and Sit, N., 2021. “Application of ultrasound in combination with other technologies in food processing: A review.” Ultrason. Sonochem., 73, 105506.

Syahir, A., Sulaiman, S., Mel, M., Othman, M., and Zubaidah Sulaiman, S., 2020. “An overview: Analysis of ultrasonic-assisted extraction’s parameters and its process.” IOP Conf. Ser. Mater. Sci. Eng., 778, 12165.

Tanaka, S., Seki, M., Miki, T., Umemura, K., and Kanayama, K., 2017. “Solute diffusion into cell walls in solution-impregnated wood under conditioning process III: effect of relative humidity schedule on solute diffusion into shrinking cell walls.” J. Wood Sci., 63, 263–270.

Tohidi, B., Rahimmalek, M., and Arzani, A., 2017. “Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran.” Food Chem., 220, 153–161.

Zhang, L., Ravipati, A.S., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Smith, P.T., Bartlett, J., Shanmugam, K., Münch, G., and Wu, M.J., 2011. “Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds.” J. Agric. Food Chem., 59, 12361–12367.

Zhang, Q.-W., Lin, L.-G., and Ye, W.-C., 2018. “Techniques for extraction and isolation of natural products: a comprehensive review.” Chin. Med., 13, 20.


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