Sterilisasi Peralatan dan Media Kultur Jaringan

https://doi.org/10.22146/a.77010

Sri Wulandari(1*), Yonita Sholihatun Nisa(2), Taryono Taryono(3), Siwi Indarti(4), Rahmi Sri Sayekti(5)

(1) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(2) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(3) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(4) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(5) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Plant tissue culture is a technology used to propagate plants under controlled and aseptic environmental conditions. One of the obstacles in tissue culture activities is the presence of bacterial and fungal contamination. Equipment and growing media that are used for tissue culture can be the source of contamination. The method that can be used to reduce contamination is using sterilization. Sterilization is used to kill or eliminate microorganisms so that the equipment and media are free from contaminants. Equipment sterilization can be done through several methods such as wet sterilization and dry sterilization. Wet sterilization is carried out using an autoclave, while dry sterilization can be carried out using an oven, fire, and glass bead sterilizer. Media sterilization is generally carried out using an autoclave or membrane filter.


Keywords


plant tissue culture, source of contaminant, sterilization, equipment, culture media

Full Text:

PDF


References

Alkhadim, S. A. S. (2018). Hot air oven for sterilization: definition and working principle. SSRN Electronic Journal, 1-7.

Bhojwani, S.S. and P.K. Dantu. (2013). Plant Tissue Culture: And Introductory Text. Springer, India.

Gaikwad, A.V., S.K. Singh, and R. Gilhotra. (2017). Plant tissue culture – a review. Journal of Pharmaceutical Research and Education 2(1), 217 – 220.

Gupta, N.V. and Shukshith K.S. (2016). Qualification of autoclave. International Journal of PharmTech Research 9(4), 220-226.

Ikenganyia, E.E., M.A.N. Anikwe, T. E. Omeje, and J. O. Adinde. (2017). Plant tissue culture regeneration and aseptic techniques. Asian Journal of Biotechnology and Bioresource Technology 1(3), 1-6.

Jain, (2016). Plant Tissue Culture Lab Practices Made Easy (For Beginners). Maharaja Ranjit International E Publication, Indore.

Misra, A.N. and M. Misra. (2012). Sterilization Techniques in Plant Tissue Culture. Fakir Mohan University, Balasore.

Rogers, W. J. (2012). Steam and dry heat sterilization of biomaterials and medical devices. Sterilization of Biomaterials and Medical Devices, 20–55.



DOI: https://doi.org/10.22146/a.77010

Article Metrics

Abstract views : 2438 | views : 3060

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.