Perkecambahan Polen Bunga Jengger Ayam (Celosia argantea)

https://doi.org/10.22146/a.77012

Nafila Alifia Azka(1*)

(1) Pusat Inovasi Agroteknologi, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Celosia cristata is one of specialty cut flowers category. Celosia cristata has leaf color, leaf shape and stem color diversity. The diversity in Celosia cristata has attracted the attention of breeders to develop new varieties of this commodity by means of hybridization. Therefore, before the hybridization of Celosia cristata is being conducted, it is necessary to study the estimated time required for pollen to reach the embryo sac and fertilization occurs to ensure the probability of successful hybridization in plant breeding program. Pollen germination in this study was carried out by in vitro method using pollen germination media consisting of 12 grams of sucrose, 0.01 grams of H3BO3, 100 ml of distilled water, and 0.1 M HCl or 0.1 M NaOH (to maintain the pH of the media at pH 6.4). The germinated pollen was observed with a microscope until the germination reed reached its maximum length, then the length of the germination reed was measured. The measured growth of germination reeds was then compared with the length of the distance from the stigma to the embryo sac. After that, the estimated time required from pollination to fertilization was being predicted. Based on the results of the study, the estimated time needed by Celosia cristata from pollination (pollen fall to the stigma) to fertilization (fusion between male and female gamete cells) was 131.9 minutes.


Keywords


Celosia cristata, in vitro, pollen germination, fertilization

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References

Anonim. 2014. Pedoman Teknis Kegiatan Peningkatan Produktivitas dan Mutu Produk Produktivitas dan Mutu Produk Florikultura Berkelanjutan Tahun 2014. Direktorat Jenderal Hortikultura Kementerian Pertanian, Jakarta.

Balasubrahmanyam A., V.K. Baranwal, M.L. Lodha, A. Varma, dan H.C. Kapoor. 2000. Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata. Plant Sci. 154: 13–21.

Hu, H. and J.Z. Zeng. 1984. Development of new varieties via anther culture. In: Handbook of Plant Cell Culture Vol.3. Macmillan Co., New York.

Kedar, N. A. and C. G. Clayton. 1998. In vitro germination and viability of buckwheat (Fagopyrum esculentum Moench) pollen. Euphytica 102(1): 87-92.

Keller, W.A., T. Rajhathy, and J. Lacapra. 1975. In vitro production of plants from pollen in Brassica campestris. CN. J. Genet. Cytol. 17:655-666.

Mastuti R., E.L Arumningtyas, dan A.A. Fatinah. 2013. Profile of some celosia variants in East Java. Dalam: Lilibeth, G. Elbers, S.K. Lai, L.K. Ryeol, D. Mallet, L. Morawska, P. Solich, M. Suzuki, H. Tsuboi, dan J.L. Wang, (Eds).

Basic Ortiz M.A., K. Hyrczyk, dan R.G. Lopez. 2012. Comparison of high tunnel and field production of specialty cut flowers in the midwest. HortScience 47(9): 12651269.

Perveen, A. 2007. Pollen germination capacity, viability and Maintanence of Pisum sativum L. papilionaceae). Middle-East Journal of Scientific Research 2: 79-81.

Sejati, R.W. 2010. Studi Jenis dan Populasi Serangga-serangga yang Berasosiasi dengan Tanaman Berbunga pada Pertanaman Padi. Skripsi. Fakultas Pertanian Universitas Sebelas Maret, Surakarta.

Shaikh, T., V.D. Devarkar, and S. Sangaekar. 2018. Phytochemical and taxonomical studies of Celosia argantea L. (Amaranthaceae). International Journal of Scientific Research in Science and Technology 4(5):481-486.

Wang, Q. L. Lu, X. Wu, Y. Li and J. Lin. 2003. Boron influences pollen germination and pollen tube growth in Picea meyeri. Tree Physiology 23: 345-351.

Widiastuti, A. dan E. R. Palupi. 2008. Viabilitas serbuk sari dan pengaruhnya terhadap keberhasilan pembentukan buah kelapa sawit (Elaeis guineensis Jacq.). Jurnal Biodiversitas 1(9): 35-38.



DOI: https://doi.org/10.22146/a.77012

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