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ISOLASI DAN PEMANFAATAN BAKTERI PROTEOLITIK UNTUK MEMPERBAIKI KUALITAS LIMBAH CAIR PENGOLAHAN BANDENG PRESTO (Isolation and Utilization of Proteolytic Bacteria to Improve The Quality of Milkfish Presto Processing Wastewate)

https://doi.org/10.22146/jml.18558

Riky Paskandani(1*), Ustadi Ustadi(2), Amir Husni(3)

(1) Jurusan Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Jl. Flora Gedung A4 Bulaksumur Yogyakarta 55281
(2) Jurusan Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Jl. Flora Gedung A4 Bulaksumur Yogyakarta 55281
(3) Jurusan Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Jl. Flora Gedung A4 Bulaksumur Yogyakarta 55281
(*) Corresponding Author

Abstract


ABSTRAK

Industri pengolahan ikan menghasilkan limbah dengan kadar protein tinggi. Penambahan bakteri proteolitik diharapkan dapat membantu mendegradasi limbah berprotein tinggi. Pada penelitian ini, isolasi bakteri proteolitik dilakukan untuk mendapatkan isolat dengan aktivitas proteolitik tinggi. Isolat terbaik diuji kemampuan hidup dan aktivitasnya pada berbagai konsentrasi NaCl dan pH. Kemampuan isolat meningkatkan kualitas limbah juga diuji pada limbah pengolahan bandeng presto. Hasil penelitian diperoleh 36 isolat bakteri proteolitik dari limbah cair pencucian ikan Pasar Kranggan Yogyakarta. Dari ke-36 isolat tersebut, isolat D61 merupakan isolat dengan aktivitas proteolitik tertinggi dengan zone diameter 20,5 mm. Isolat tersebut mampu hidup pada NaCl 0-10% dan kisaran pH 5-10, namun aktivitas proteolitik tertingginya pada NaCl 0-2% dan kisaran pH 7-8 dan pH 10. Berdasarkan karakteristik morfologi dan biokimianya, isolate D61 memiliki kemiripan 93,93% dengan Bacillus soli. Hasil ujicoba pada limbah cair pengolahan bandeng presto ternyata isolat D61 tidak mampu memperbaiki kualitas limbah tersebut.

ABSTRACT

In this research, isolation of proteolytic bacteria was performed to obtain isolates with high proteolytic activity based on their ability to degrade the protein. The best isolate was examined for their growth ability and proteolytic activity in different concentrations of NaCl and degree of acidity (pH). Furthermore, the isolate ability to improve wastewater quality was observed in milkfish presto processing wastewater. Thirty-six proteolytic bacteria were isolated from fish washing wastewater of Kranggan Market, Yogyakarta. Isolate D61 has the highest proteolytic activity with activity about 20,5 mm. Isolate D61 was able to grow at a concentration of 0-10% NaCl with the highest proteolytic activity at the concentration of 0-2% NaCl. Isolate D61 was also able to live at pH 5-10 with the highest proteolytic activity at pH 7-8 and pH 10. Based on morphology and biochemical characteristics, D61 has 93.93% similarity with Bacillus soli. However, isolate D61 was not able to improve the quality of milkfish presto processing wastewater.


Keywords


Bacillus soli; bakteri proteolitik; bandeng presto; limbah cair; pengolahan limbah; milkfish presto; Proteolytic bacteria; wastewater; water treatment



References

Anonim, 2004. SNI No. 06-6989.11-2004: Air dan Air Limbah - Bagian 11 - Cara Uji Derajat Keasaman (pH) dengan Menggunakan Alat pH Meter. Badan Standarisasi Nasional (BSN). Jakarta.

Anonim, 2005. Standard Methods for the Examination of Water & Wastewater. 21th edition. American Public Health Association. New York. p. 1368

Anonim, 2006. SNI No. 01-2332.3.2006: Cara Uji Mikrobiologi-Bagian 3: Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan. Badan Standarisasi Nasional (BSN). Jakarta.

Boopathy, R., 2000. Review Paper: Factor Limiting Bioremediation Technologies. Biores. Technol. 75:63-67.

Carawan, R.E., 1991. Processing Plant Waste Management Guidelines for Aquatic Fishery Products. Food and the Environment. Website : http://www.p2pays.org/ref/02/01796.pdf. Diakses pada 30 September 2012.

Chowdhury, P., Viraraghavan, T., dan Srinivasan, A., 2010. Biological Treatment Processes for Fish Processing Wastewater – A Review. Biores. Technol. 101:439-449.

Dajanta, K., Wongkham, S., Thirach, P., Baophoeng, P., Apichartsrangkoon, A., Santithum, P., dan Chukeatirote, E., 2009. Comparative Study of Proteolytic Activity of Protease-Producing Bacteria Isolated from Thua nao. Maejo. Int. J. Sci. & Technol. 3(02):269-276.

De Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.H., dan Whitman, W.B., 2009. Bergey’s Manual of Systematic Bacteriology. 2nd Ed.. Vol. 3 : Firmicutes. Springer. Washington DC.

Fong, K.P.Y., dan Tan, H.M., 2000. Isolation of a Microbial Consortium from Activated Sludge for the Biological Treatment of Food Waste. World J. Microb. & Biotechnol. 16:441-443.

Gardini, F., Martuscelli, M., Caruso, M.C., Galgano, F., Crudele, M.A., Favati, F., Guerzoni, M.E., dan Suzzi, G., 2001. Effects of pH, Temperature and NaCl Concentration on the Growth Kinetics, Proteolytic Activity and Biogenic Amine Production of Enterococcus faecalis. Int. J. Food Microb. 64:105-117.

Gonzalez, J.F., 1996. Wastewater Treatment in the Fishery Industry. FAO Fisheries Technical Paper – 355. Food and Agriculture Organization. Amerika.

Heyrman, J., Vanparys, B., Logan, N.A., Balcaen, A., Rodriguez-Diaz, M., Felske, A., dan De Vos, P., 2004. Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A Grasslands. Int. J. Systematic & Evolutionary Microb. 54:47-57.

Isnansetyo, A., dan Kamei, Y., 2009. Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Activity of MC21-B, an Antibacterial Compound Produced by the Marine Bacterium Pseudoalteromonas phenolica O-BC30T. Int. J. Antimicrobial Agents 34(2):131-135.

Kim, J.K., Dao, V.T., Kong, I.S., dan Lee, H.H., 2010. Identification and Characterization of Microorganisms from Earthworm Viscera for the Conversion of Fish Wastes into Liquid Fertilizer. Biores. Technol. 101:5131-5136.

Loperena, L., Ferrari, M.D., Saravia, V., Murro, D., Lima, C., Ferrando, L., Fernández, A., dan Lareo, C., 2007. Performance of a Commercial Inoculum for the Aerobic Biodegradation of a High Fat Content Dairy Wastewater. Biores. Technol. 98:1045–1051.

Loperena, L., Ferrari, M.D., Diaz, A.L., Ingold, G., Perez, L.V., Carvallo, F., Travers, D., Menes, R.J., dan Lareo, C., 2009. Isolation and Selection of Native Microorganisms for the Aerobic Treatment of Simulated Dairy Wastewater. Biores. Technol.. 100:1762-1766.

Mohan, T.S., Polavedam, A., dan Immanvel, G., 2008. Isolation and Characterization of Lipase-producing Bacillus strains from Oil Mill Waste. Afr. J. Biotechnol. 15:2728-2735.

Muchtadi, D. dan Betty, S.L., 1983. Petunjuk Praktek Mikrobiologi Hasil Perikanan 2. Departemen Pendidikan dan Kebudayaan. Jakarta.

Nelson, D.L., dan Cox, M.M., 2004. Lehninger’s Principles of Biochemistry, 4th Edition. W. H. Freeman and Company, New York.

Prescott, L.M., Harley, J.P., dan Klein, D.A., 2002. Microbiology. 5th Edition. McGraw-Hill. London.

Schlegel, H.G. dan Schmidt, K., 1994. Mikrobiologi Umum. Edisi keenam. Gadjah Mada University Press. Yogyakarta.

Sizemore, R.K., dan Stevenson, L.H. 1970. Method for the Isolation of Proteolytic Marine Bacteria. Appl. Microb. 20(6):991-992.

St Leger, R.J., Joshi, L., dan Roberts, D., 1998. Ambient pH is a Major Determinan in the Expression of Cuticle-degrading Enzymes and Hydrophobin by Metarhizium nisopliae. Appl. & Env. Microb. 64:709-713.

Tarntip, R., dan Sirichom, T., 2011. Isolation of Proteolytic, Lipolytic, and Bioemulsifying Bacteria for Improvement of the Aerobic Treatment of Poultry Processing Wastewater. Afr. J. Microb. Res. 5(30):5493-5497.

Urano, N., Sasaki, E., Ueno, R., Namba, H., dan Shida, Y., 2002. Bioremediation of Fish Cannery Wastewater with Yeast Isolated from a Drainage Canal. Marine Biotechnol. 4:559-564.

Watanabe, K., Miyashita, M., dan Harayamam, S., 2000. Starvation Improves Survival of Bacteria Introduced into Activated Sludge. Appl. & Env. Microb. 66(9):3905-3910.

Xia, Z., dan Wu, S., 2012. Cell Number as an Important Variable in Optimising Inoculum Age and Size in Yeast Cultivation. Afr. J. Biotechnol. 11(4):919-922.

Yu, Z., dan Mohn, W.W., 2002. Bioaugmentation with Resin Acid-degrading Bacterium Zooloea resiniphila DhA-35 to Counteract pH Stess in an Aerated Lagoon Treating Pulp and Paper Mill Effluent. Water Resource 36:2793-2801.



DOI: https://doi.org/10.22146/jml.18558

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