SINTESIS NANOPARTIKEL DARI PERAK NITRAT DENGAN TIROSIN SEBAGAI REDUKTOR DAN AGEN PENGKAPING UNTUK MEMBENTUK NANOKOMPOSIT FILM AgNPs-POLI ASAM LAKTAT SEBAGAI ANTIBAKTERI



Chuchita Chuchita(1*)

(1) Gadjah Mada University
(*) Corresponding Author

Abstract


Telah dilakukan sintesis nanopartikel dari perak nitrat dengan asam amino tirosin sebagai reduktor dan agen pengkaping. Reaksi dilakukan pada berbagai pH dan konsentrasi tirosin untuk mengetahui pH dan konsentrasi optimum tirosin dan di analisis dengan instrumen spektrofotometer UV-Vis. Variasi pH tirosin yaitu pada pH 7-13, dan variasi konsentrasi tirosin masing-masing 0,02 mM, 0,1 mM, 0,5 mM, 1 mM, 5 mM, 10 mM, 15 mM, dan 20 mM. Variasi waktu reaksi juga diteliti untuk mengetahui waktu reaksi optimum untuk sintesis AgNPs. Variasi waktu reaksi yang digunakan adalah 15, 30, 45, 60, 75, 90, 105, dan 120 menit. AgNPs disintesis untuk membentuk nanokomposit pada film poli asam laktat (PLA). Nanokomposit film PLA nanopartikel perak (KAgNPs) ini kemudian digunakan sebagai antibakteri.  Karakterisasi dilakukan dengan instrumen spektrofotometer UV-Vis, FTIR, TEM, dan SEM EDX.

Hasil eksperimen menunjukkan bahwa asam amino tirosin dapat berperan sebagai reduktor dan agen penkaping dalam sintesis AgNPs ditandai dengan perubahan warna dari tidak berwarna menjadi kuning. pH sistem berpengaruh terhadap kemampuan tirosin dalam mereduksi ion perak. AgNPs terbentuk pada keadaan basa dengan pH optimum 11. Konsentrasi optimum tirosin adalah 1 mM dan konsentrasi AgNO3 1 mM, sehingga didapatkan perbandingan konsentrasi asam amino tirosin dan AgNO3 yaitu 1:1. Waktu reaksi optimum untuk mensintesis AgNPs terjadi pada menit ke empat puluh lima pada suhu 100 oC.  Hasil karakterisasi AgNPs menggunakan TEM menunjukan bahwa pertikel AgNPs berbentuk bulat. Ukuran partikel rata-rata AgNPs dalam koloidalnya adalah 15,10 nm, sedangkan nanokomposit KAgNPs memiliki ukuran partikel rata-rata yang lebih besar yaitu 15,30 nm.

Kata kunci: nanopartikel perak, tirosin, antibakteri.


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