Identification of Protease Producing Halophilic Bacteria from Bledug KuwuMud Volcano
https://doi.org/10.22146/ijbiotech.15995 Muhammad Saifur Rohman(1*), Irfan Dwidya Prijambada(2), Yohanna Anisa Indriyani(3), Heri Hendrosatriyo(4)
(1) Laboratory of Soil and Environmental Microbiology, Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Laboratory of Soil and Environmental Microbiology, Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Laboratory of Soil and Environmental Microbiology, Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Laboratory of Soil and Environmental Microbiology, Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
The objective of this research was to isolate and identify the halophilic bacteria from Bledug Kuwu-mud
volacano having an ability to produce proteolytic enzyme. From this work, 6 bacterial isolates were obtained
from mud and water samples using artificial sea water media after incubation at room temperature. Three
out of the 6 isolates (BKL-3, BKL-5, and BKA-1) were selected for further analysis. BKL-3, BKL-5 and BKA-1
exhibited an ability to grow at salt concentration greater than 10%. BKL-3 could grow on media supplemented
with 15% of salt, meanwhile BKL-5 and BKA-1 could grow at 20% of salt, respectively. Furthermore, those
isolates also exhibited proteolytic activity when they were grown on casein media. The phylogenetic analysis
based on the 16SrRNA gene sequences showed that the BKL-3 belong to the group of Bacillaceae, whilst BKL-5
and BKA-1 isolates were relatively distance from the group of Halomonadaceae. Therefore, BKL-5 and BKA-1
could be considered as the allegedly new species that were separated from Halomonadaceae
volacano having an ability to produce proteolytic enzyme. From this work, 6 bacterial isolates were obtained
from mud and water samples using artificial sea water media after incubation at room temperature. Three
out of the 6 isolates (BKL-3, BKL-5, and BKA-1) were selected for further analysis. BKL-3, BKL-5 and BKA-1
exhibited an ability to grow at salt concentration greater than 10%. BKL-3 could grow on media supplemented
with 15% of salt, meanwhile BKL-5 and BKA-1 could grow at 20% of salt, respectively. Furthermore, those
isolates also exhibited proteolytic activity when they were grown on casein media. The phylogenetic analysis
based on the 16SrRNA gene sequences showed that the BKL-3 belong to the group of Bacillaceae, whilst BKL-5
and BKA-1 isolates were relatively distance from the group of Halomonadaceae. Therefore, BKL-5 and BKA-1
could be considered as the allegedly new species that were separated from Halomonadaceae
Keywords
halophilic; halotolerant; protease; 16SrRNA gene; Bledug Kuwu-mud volcano; phylogenetic tree
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PDF DOI: https://doi.org/10.22146/ijbiotech.15995
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Copyright (c) 2016 Muhammad Saifur Rohman, Irfan Dwidya Prijambada, Yohanna Anisa Indriyani, Heri Hendrosatriyo
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