Determination of Haemaglutinin and Gene Encoding Fibronectin Binding Proteins Staphylococcus aureus Isolated from Dairy Milk Cows
https://doi.org/10.22146/ijbiotech.16367 Feny Prabawati Pratomo(1), Siti Isrina Oktavia Salasia(2*), Syarifudin Tato(3)
(1) Research Center for Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Departement of Pharmacology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Staphylococcus aureus is a major pathogen causing clinical and subclinical mastitis in dairy milk cows. The
mastitis has immense economical impacts, where it reduces of the quantity and quality of milk production.
The aims of the research were to analyse haemaglutinin and gene encoding fbronectin binding proteins.
Nineteen Staphylococcus aureus isolates used in the present study were isolated from dairy milk cows from
Yogyakarta, Solo, Boyolali and Sumedang. The haemagluitinin of S. aureus were determined based on
haemaglutination reaction to erythrocytes of rabbit. Detection of gene encoding fbronectin binding proteins
could be performed with specifc primers using polymerase chain reaction (PCR). The results of studies
showed that most of S. aureus (78,95%) expressed haemaglutinin based on their ability to aglutinate rabbit
erythrocytes. Analysis of gene encoding fbronectin binding proteins of S. aureus revealed gene fnbA with
size of approximately 1300 bp for 57,89% isolates, gene fnbB with size of approximately 900 bp for 31,58%
isolates and both of gene fnbA and fnbB could be detected for 31,58% isolates. The characters of S. aureus
based on haemaglutinin, gene fnbA and fnbB of the present study could be used as an information to control
of S. aureus infection in dairy herds.
mastitis has immense economical impacts, where it reduces of the quantity and quality of milk production.
The aims of the research were to analyse haemaglutinin and gene encoding fbronectin binding proteins.
Nineteen Staphylococcus aureus isolates used in the present study were isolated from dairy milk cows from
Yogyakarta, Solo, Boyolali and Sumedang. The haemagluitinin of S. aureus were determined based on
haemaglutination reaction to erythrocytes of rabbit. Detection of gene encoding fbronectin binding proteins
could be performed with specifc primers using polymerase chain reaction (PCR). The results of studies
showed that most of S. aureus (78,95%) expressed haemaglutinin based on their ability to aglutinate rabbit
erythrocytes. Analysis of gene encoding fbronectin binding proteins of S. aureus revealed gene fnbA with
size of approximately 1300 bp for 57,89% isolates, gene fnbB with size of approximately 900 bp for 31,58%
isolates and both of gene fnbA and fnbB could be detected for 31,58% isolates. The characters of S. aureus
based on haemaglutinin, gene fnbA and fnbB of the present study could be used as an information to control
of S. aureus infection in dairy herds.
Keywords
Staphylococcus aureus; haemaglutinin; gene encoding fbronectin binding protein; milk cow
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PDF DOI: https://doi.org/10.22146/ijbiotech.16367
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