Decolorization and detoxification of batik dye effluent containing Indigosol Blue-04B using fungi isolated from contaminated dye effluent

Ratna Stia Dewi(1*), Rina Sri Kasiamdari(2), Erni Martani(3), Yekti Asih Purwestri(4)

(1) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia; Faculty of Biology, Universitas Jenderal Soedirman, Jalan Dr. Soeparno No. 63, Purwokerto 53122, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Agriculture Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora, Bulaksumur, Yogyakarta 55281, Indonesia
(4) Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia; Research Center for Biotechnology, Universitas Gadjah Mada, Jalan Teknika Utara, Sleman, Yogyakarta 55281, Indonesia
(*) Corresponding Author


Fungi are capable of treating various synthetic dye effluents. Previously, we isolated seven strains of fungi from contaminated batik dye effluent at Banyumas, Central Java. The aims of this study were to screen the ability of these fungi to decolorize batik dye effluents containing Indigosol Blue-04B and to investigate the phytotoxicity effects of biodegraded effluent on the germination of corn seeds Zea mays L. and green bean seeds Vigna radiata (L.) Wilczek. In addition, the decolorized effluents were tested for toxic effect on the agriculturally important gram-positive and gram-negative soil bacteria Bacillus cereus and Azotobacter sp., Staphylococcus aureus and Escherichia coli, respectively. Study of decolorization showed that fungi were able to decolorize Indigosol Blue-04B batik dye effluents by 21.04% to 99.89% at room temperature after three days of incubation. The assay of phytotoxicity showed that both plumule and radicle length of Z. mays and V. radiata grown on the decolorized effluent was longer than on untreated effluent. The percentage of Z. mays and V. radiata seed germination in decolorized effluent was higher than in untreated effluent. There was no inhibition zone found around the decolorized effluent samples after incubating the bacteria for 48 hours. Aspergillus sp. 3 was the most effective for degradation and could be used for batik effluent mycoremediation processes.


batik dye effluents; decolorization; Indigosol Blue-04B; microbial toxicity; phytotoxicity

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Abadulla E, Robra KH, Gübitz GM, Silva LM, CavacoPaulo A. 2000. Enzymatic decolorization of textile dyeing effluents. Text Res J 70(5):409–414. doi:10.1177/004051750007000506.

AI-Jawhari IFH. 2015. Decolorization of methylene blue and crystal violet by some filamentous fungi. Int J Environ Biorem Biodegrad 3(2):62–65. doi:10.12691/ijebb-3-2-4.

Aksu Z, Çalik A, Dursun A, Demircan Z. 1999. Biosorption of iron(III)–cyanide complex anions to Rhizopus arrhizus: application of adsorption isotherms. Process Biochem 34(5):483–491. doi:10.1016/s0032- 9592(98)00115-0.

Aksu Z, Tezer S. 2000. Equilibrium and kinetic modelling of biosorption of Remazol Black B by Rhizopus arrhizus in a batch system: effect of temperature. Process Biochem 36(5):431–439. doi:10.1016/s0032- 9592(00)00233-8.

Arora DS, Chander M. 2004. Decolourisation of diverse industrial dyes by some Phlebia spp. and their comparison with Phanerochaete chrysosporium. J Basic Microbiol 44(5):331–338. doi:10.1002/jobm.200410391.

Balan DSL, Monteiro RTR. 2001. Decolourisation of textile dyes indigo dye by lignolytic fungi. J Biotechnol 89(2-3):141–145. doi:10.1016/s0168-1656(01)00304- 2.

Braun S, Vecht-Lifshitz SE. 1991. Mycelial morphology and metabolite production. Trends Biotechnol 9(2):63–68. doi:10.1016/0167-7799(91)90191-j.

Campos R, Cavaco-Paulo A, Robra KH, Schneider M, Gübitz G. 2001a. Indigo degradation with laccases from Polyporus sp. and Sclerotium rolfsii. Text Res J 71(5):420–424. doi:10.1177/004051750107100509.

Campos R, Kandelbauer A, Robra K, Cavaco-Paulo A, Gübitz G. 2001b. Indigo degradation with purified laccases from Trametes hirsuta and Sclerotium rolfsii. J Biotechnol 89(2-3):131–139. doi:10.1016/s0168- 1656(01)00303-0.

Coulibaly L, Gourene G, Agathos NS. 2003. Utilization of fungi for biotreatment of raw wastewaters. Afr J Biotechnol 2(12):620–630. doi:10.5897/ajb2003.000- 1116.

Dewi RS, Kasiamdari RS, Martani E, Purwestri YA. 2016. Studi komparatif penurunan warna limbah cair batik menggunakan Aspergillus niger[comparative study of color decrease of batik liquid waste using Aspergillus niger]. In: Prosiding Symbion (Symposium on Biology Education). p. 269–277.

Ferreira VS, Magalhães DB, Kling SH, Silva JGD, Bon EP. 2000. N-demethylation of methylene blue by lignin peroxidase from Phanerochaete chrysosporium. Appl Biochem Biotechnol 84–86:255–265. doi:10.1007/978-1-4612-1392-5_19.

Fu Y, Viraraghavan T. 2001. Fungal decolorization of dye wastewaters: a review. Bioresour Technol 79. doi:10.1016/s0960-8524(01)00028-1.

Ilyas S, Rehman A. 2013. Decolorization and detoxification of Synozol red HF-6bn azo dye, by Aspergillus niger and Nigrospora sp. Iran J Environ Health Sci Eng 10(1):12. doi:10.1186/1735-2746-10-12.

Kariyajjanavar P, Narayana J, Nayaka YA. 2013. Electrochemical degradation of C.I. Vat Orange 2 dye on carbon electrode. Inventi Impact: Water Environ 2013(3):106–112.

Kaushik P, Malik A. 2009. Fungal dye decolourization: recent advances and future potential. Environ Int 35(1):127–141. doi:10.1016/j.envint.2008.05.010.

Knapp JS, Newby PS. 1995. The microbiological decolorization of an industrial effluent containing a diazolinked chromophore. Water Res 29(7):1807–1809. doi:10.1016/0043-1354(94)00341-4.

Kurniasih M, Dewi RS, Purwati P, Hermawan D, AboulEnein HY. 2018. Synthesis, characterization and antifungal activity of N-methyl chitosan and its application on the gauze. Curr Bioact Compd 14(4):347–356. doi:10.2174/1573407213666170420171005.

Mahmood R, Sharif F, Ali S, Hayyat MU. 2015. Enhancing the decolorizing and degradation ability of bacterial consortium isolated from textile effluent affected area and its application on seed germination. Sci World J 2015:1–9. doi:10.1155/2015/628195.

Manu B, Chaudhari S. 2003. Decolorization of indigo and azo dyes in semicontinuous reactors with long hydraulic retention time. Process Biochem 38(8):1213– 1221. doi:10.1016/s0032-9592(02)00291-1.

Naeem Ali MFSPBGSA Abdul Hameed. 2009. Application of Aspergillus niger SA1 for the enhanced bioremoval of azo dyes in simulated textile effluent. Afr J Biotechnol 8(16):3839–3845.

Parshetti GK, Kalme SD, Gomare SS, Govindwar SP. 2007. Biodegradation of reactive blue-25 by Aspergillus ochraceus NCIM1146. Bioresour Technol 98(18):3638–3642. doi:10.1016/j.biortech.2006.11.017.

Pitt JI, Hocking AD. 2009. Fungi and food spoilage. New York: Springer. doi:10.1007/978-0-387-92207-2.

Rani B, Kumar V, Singh J, Bisht S, Teotia P, Sharma S, Kela R. 2014. Bioremediation of dyes by fungi isolated from contaminated dye effluent sites for bio-usability. Braz J Microbiol 45(3):1055–1063. doi:10.1590/s1517-83822014000300039.

Sumathi S, Manju BS. 2000. Uptake of reactive textile dyes by Aspergillus foetidus. Enzyme Microb Technol 27(6):347–355. doi:10.1016/s0141-0229(00)00234- 9.

Sunarto. 2008. Teknologi pencelupan dan pengecapan [Technology of dyeing and application]. Jakarta: Departemen Pendidikan Nasional Indonesia.

Tian Ce, Tian R, Zhou Y, Chen Q, Cheng H. 2013. Decolorization of indigo dye and indigo dye-containing textile effluent by Ganoderma weberianum. Afr J Microbiol Res 7(11):941–947. doi:. 904.

Watharkar AD, Jadhav JP. 2014. Detoxification and decolorization of a simulated textile dye mixture by phytoremediation using Petunia grandiflora and Gailardia grandiflora: A plant–lant consortial strategy. Ecotoxicol Environ Saf 103:1–8. doi:10.1016/j.ecoenv.2014.01.033.

Wesenberg D, Kyriakides I, Agathos SN. 2003. Whiterot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 22(1-2):161–187. doi:10.1016/j.biotechadv.2003.08.011.

Wong Y, Yu J. 1999. Laccase-catalyzed decolorization of synthetic dyes. Water Res. 33(16):3512–3520. doi:10.1016/s0043-1354(99)00066-4.

Zheng Z, Levin RE, Pinkham JL, Shetty K. 1999. Decolorization of polymeric dyes by a novel Penicillium isolate. Process Biochem 34:31–37. doi:10.1016/S0032- 9592(98)00061-2.

Zhou W, Zimmermann W. 1993. Decolorization of industrial effluents containing reactive dyes by actinomycetes. FEMS Microbiol Lett 107(2-3):157–161. doi:10.1111/j.1574-6968.1993.tb06023.x.


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