Phenotype of Transgenic Tobacco Plants (Nicotiana tabacum cv. Petit Havana SR-1) Expressing 1724orf13 Gene of Agrobacterium rhizogenes strain MAFF301724
https://doi.org/10.22146/ijbiotech.7771 Niken Satuti Nur Handayani(1*), Nobukazu Tanaka(2), Kazuo Yoshida(3)
(1)
(2)
(3)
(*) Corresponding Author
Abstract
Nicotiana tabacum cv. Petit Havana SR-1 transgenic plants expressing ORF13 of Agrobacterium rhizogenes strain
MAFF301724 under different promoters displayed plant morphology abnormalities. They were small, with short
and variable internodes lengths; leaves were small, asymmetric, rounded, wrinkled and dark green; flowers were
short, and irregularly shaped. This phenotype was also exhibited, similar, but not completely the same, to those of
hairy root syndrome, indicating that expression of ORF13 influences plant development.
Keywords: ORF13, Agrobacterium rhizogenes strain MAFF301724, transgenic plants, morphology abnormalities
MAFF301724 under different promoters displayed plant morphology abnormalities. They were small, with short
and variable internodes lengths; leaves were small, asymmetric, rounded, wrinkled and dark green; flowers were
short, and irregularly shaped. This phenotype was also exhibited, similar, but not completely the same, to those of
hairy root syndrome, indicating that expression of ORF13 influences plant development.
Keywords: ORF13, Agrobacterium rhizogenes strain MAFF301724, transgenic plants, morphology abnormalities
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PDF DOI: https://doi.org/10.22146/ijbiotech.7771
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