T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1
https://doi.org/10.22146/ijbiotech.7776 Muhammad Da’i(1*), Umar Anggara Jenie(2), Supardjan AM(3), Masashi Kawaichi(4), Edy Meiyanto(5)
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(*) Corresponding Author
Abstract
its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticancer
activities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxic
effects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progression
was carried out using flowcytometry. Western blot analysis was used to analyze protein expression corresponding
to cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycle
progression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation induced
by PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIP
through p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated by
PARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibits
the growth of T47D cells targeted on microtubules.
Keywords: PGV-1, G2/M arrest, apoptosis, p21
activities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxic
effects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progression
was carried out using flowcytometry. Western blot analysis was used to analyze protein expression corresponding
to cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycle
progression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation induced
by PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIP
through p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated by
PARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibits
the growth of T47D cells targeted on microtubules.
Keywords: PGV-1, G2/M arrest, apoptosis, p21
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PDF DOI: https://doi.org/10.22146/ijbiotech.7776
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