Improving transient gene expression and agroinfiltration‐based transformation effectiveness in Indonesian orchid Phalaenopsis amabilis (L.) Blume
Dionysia Heviarie Primasiwi(1), Yekti Asih Purwestri(2), Endang Semiarti(3*)
(1) Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Indonesia
(3) Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Indonesia
(*) Corresponding Author
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Din Mufti FU, Aman S, Banaras S, Shinwari ZK, Shakeel S. 2015. ACTIN gene identification from selected medicinal plants for their use as internal controls for gene expression studies. Pakistan J. Bot. 47(2):629– 635.
Dwiyani R, Yuswanti H, Sartika Mercuriani I, Semiarti DE. 2016. Transformasi gen pembungaan melalui Agrobacterium tumefaciens secara in vitro pada tanaman anggrek Vanda tricolor [In vitro transformation of flowering genes via Agrobacterium tumefaciens in Vanda tricolor orchid plants]. AGROTROP 6(1):83– 89.
Faizal A, Geelen D. 2012. Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata. Plant Cell Rep. 31(8):1517–1526. doi:10.1007/s00299 01212664.
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DOI: https://doi.org/10.22146/ijbiotech.80555
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