Antimicrobial compounds from intracellular and extracellular secondary metabolites of Actinobacteria InaCC A759

Maya Dian Rakhmawatie; Mustofa Mustofa; Puspita Lisdiyanti; Tri Wibawa; Kanti Ratnaningrum; Muhammad Mucharom Chairul Umam; Muhammad Hasan Alfi; Listya Chariri

doi:10.22146/ijbiotech.82376

Antimicrobial compounds from intracellular and extracellular secondary metabolites of Actinobacteria InaCC A759

https://doi.org/10.22146/ijbiotech.82376

Maya Dian Rakhmawatie^(1*), Mustofa Mustofa⁽²⁾, Puspita Lisdiyanti⁽³⁾, Tri Wibawa⁽⁴⁾, Kanti Ratnaningrum⁽⁵⁾, Muhammad Mucharom Chairul Umam⁽⁶⁾, Muhammad Hasan Alfi⁽⁷⁾, Listya Chariri⁽⁸⁾

(1) Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang, Jl. Kedung Mundu Raya No.18, Semarang 50273, Indonesia
(2) Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako Sekip Utara, Yogyakarta 55281, Indonesia
(3) Research Center for Biotechnology, the National Research and Innovation Agency, Jl. Raya Jakarta‐Bogor Km. 46, Bogor 16911, Indonesia
(4) Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako Sekip Utara, Yogyakarta 55281, Indonesia
(5) Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang, Jl. Kedung Mundu Raya No.18, Semarang 50273, Indonesia
(6) Graduated Student, Faculty of Medicine, Universitas Muhammadiyah Semarang, Jl. Kedung Mundu Raya No. 18, Semarang 50273, Indonesia
(7) Graduated Student, Faculty of Medicine, Universitas Muhammadiyah Semarang, Jl. Kedung Mundu Raya No. 18, Semarang 50273, Indonesia
(8) Graduated Student, Faculty of Medicine, Universitas Muhammadiyah Semarang, Jl. Kedung Mundu Raya No. 18, Semarang 50273, Indonesia
(*) Corresponding Author

Abstract

The World Health Organization (WHO) has determined a list of pathogens that require the development of new antimicrobials due to resistance problems; these include Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. In addition, Mycobacterium smegmatis has been used for antimycobacterial discovery to address the increasing burden of tuberculosis. In this study, optimization of antimicrobial activity, secondary metabolite profiling, and strain identification was conducted on Actinobacteria InaCC A759. Intracellular and extracellular extracts of Actinobacteria InaCC A759 were found to have different antimicrobial activities. The minimum inhibitory concentration (MIC) values of the extract to inhibit the growth of M. smegmatis, E. coli, and P. aeruginosa were 50, 25, and 100 µg/mL (intracellular), and 25, 25, and 100 µg/mL (extracellular), respectively. However, neither extract was able to inhibit the growth of S. aureus. Metabolite profiling using High resolution‐mass spectrometry (HR‐MS) resulted in differences in the major compound between the two extracts of Actinobacteria InaCC A759, namely n‐acetyltyramine (C₁₀H₁₃NO₂/179.0945) (24.24%) (intracellular) and palmitic acid (C₁₆H₃₂O₂/273.27034) (86.92%) (extracellular). Based on molecular analysis of the 16S rRNA gene, Actinobacteria InaCC A759 is identical to the Streptomyces olivaceus strain FoRh46. The antimicrobial activity and secondary metabolites profiles of Streptomyces olivaceus InaCC A759 have not been previously reported.

Keywords

Actinobacteria; Antimicrobial agents; Metabolite profiling; N‐acetyltyramine; Palmitic acid

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DOI: https://doi.org/10.22146/ijbiotech.82376