Phylogenetic relationship of Gram Negative Bacteria of Enterobacteriaceae Family in the Positive Widal Blood Cultures based on 16S rRNA Gene Sequences
https://doi.org/10.22146/ijbiotech.8635 Sri Darmawati(1*), Langkah Sembiring(2), Widya Asmara(3), Wayan T. Artama(4), Masashi Kawaichi(5)
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(*) Corresponding Author
Abstract
The purpose of this study was to analyze the phylogenetic relationship of Gram negative bacteria (3
strains of Salmonella typhi, 1 strain of Escherichia coli, 1 strain of Serratia marcescens, and 3 strains of Enterobacter
cloacae) of Enterobacteriaceae family in positive Widal blood cultures based on 16S rRNA gene sequences. The
results respectively showed that each two 16S rRNA gene clones of Serratia marcescens KD 08.4 had a close
relationship with 16S rRNA gene of Serrratia marcescens ATCC 13880 (similarity: 99.53-99.8%), Eschericia coli
BA 30.1 with Eschericia coli ATCC 11775T (similarity: 99.38-99.67%), Salmonella typhi BA 07.4, Salmonella typhi
KD 30.4, and Salmonella typhi SA 02.2 with Salmonella typhi ATCC 19430T (similarity: 99.4-100%) as well as the
isolates of Enterobacter cloacae SA 02.1, Enterobacter cloacae BA 45.4.1, one 16S rRNA gene clone of Enterobacter
cloacae TG 03.5 with Enterobacter cloacae ATCC 23373 (similarity: 99.0-99.87%).
strains of Salmonella typhi, 1 strain of Escherichia coli, 1 strain of Serratia marcescens, and 3 strains of Enterobacter
cloacae) of Enterobacteriaceae family in positive Widal blood cultures based on 16S rRNA gene sequences. The
results respectively showed that each two 16S rRNA gene clones of Serratia marcescens KD 08.4 had a close
relationship with 16S rRNA gene of Serrratia marcescens ATCC 13880 (similarity: 99.53-99.8%), Eschericia coli
BA 30.1 with Eschericia coli ATCC 11775T (similarity: 99.38-99.67%), Salmonella typhi BA 07.4, Salmonella typhi
KD 30.4, and Salmonella typhi SA 02.2 with Salmonella typhi ATCC 19430T (similarity: 99.4-100%) as well as the
isolates of Enterobacter cloacae SA 02.1, Enterobacter cloacae BA 45.4.1, one 16S rRNA gene clone of Enterobacter
cloacae TG 03.5 with Enterobacter cloacae ATCC 23373 (similarity: 99.0-99.87%).
Keywords
Widal; Enterobacteriaceae; 16S rRNA genes
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PDF DOI: https://doi.org/10.22146/ijbiotech.8635
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