Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation
https://doi.org/10.22146/ijbiotech.8637 Tri Suwarni Wahyudiningsih(1*), Mohammad Naiem(2), Sapto Indrioko(3), Issirep Sumardi(4)
(1)
(2)
(3)
(4)
(*) Corresponding Author
Abstract
Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found in
peat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existence
is now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity within
four natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in Central
Kalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectric
focusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow
(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Mean
number of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Genetic
differentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozyme
variation was found within population (93.2%). Special attention is essential to conserve a private allele of
Got-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as found
in other populations. Selat Nusa population is expected to enhance the effective management for genetic
resources conservation of this vulnerable species in the future.
peat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existence
is now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity within
four natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in Central
Kalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectric
focusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow
(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Mean
number of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Genetic
differentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozyme
variation was found within population (93.2%). Special attention is essential to conserve a private allele of
Got-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as found
in other populations. Selat Nusa population is expected to enhance the effective management for genetic
resources conservation of this vulnerable species in the future.
Keywords
allozyme; genetic diversity; genetic differentiation; populations; Dyera lowii
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PDF DOI: https://doi.org/10.22146/ijbiotech.8637
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