The Susceptibility of katG Ser315Thr Mycobacterium tuberculosis mutant Against Anti Tuberculosis Drugs

Ning Rintiswati(1*), P. Praseno(2)

(1) Department of Microbiology, Faculty of Medicine, Universitas Gajdah Mada, Yogyakarta, Indonesia
(2) Department of Microbiology, Faculty of Medicine, Universitas Gajdah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


Mycobacterium tuberculosis resistant strains is a matter of great concern for TB control Program since no
cure for some multi drug resistance TB (MDR-TB) cases. These strains could spread around the world, make
a great challenges for control measures. Mycobacterium tuberculosis strains resistance to anti tuberculosis drugs
is mainly caused by the alteration in several genes encoding the molecular targets. Mutation of katG at codon
315 especially Ser315Thr are responsible for INH resistance in a large proportion of TB cases. The aim of this
study is to know the frequency of of katG Ser315Thr of M. tuberculosis mutant and the pattern of resistance to
Anti TB drugs. The study design was observational laboratoric. Eighty fi ve M. tuberculosis INH resistant clinical
isolates were screened for mutation of katGSer315Thr by using PCR-RFLP and DNA sequence analysis.The
results showed that katG Ser 315Thr were identifi ed in 23 (27,05%) of INH resistance isolates. Frequency of
MDR among the katG Ser315Thr mutants almost double than the non mutant. The result suggested that the
the katG Ser315Thr mutation may play important role in the development of MDR-TB.


katG Ser315Thr Mycobacterium tuberculosis mutant, frequency, anti TB drugs, Multi Drug Resistant, pattern of resistance

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