The Newly Bone Formation with Carbonate Apatite-Chitosan Bone Substitute in the Rat Tibia

https://doi.org/10.22146/theindjdentres.10065

Anne Handrini Dewi(1*), Andi Triawan(2)

(1) 
(2) 
(*) Corresponding Author

Abstract


Large bone defect still represent a major problem in orthopedics. A tissue engineering approach has been proposed where osteogenic cells, bioceramic scaffolds and growth factors can play in a role to the bone repair. Bone consist a mineral phase such as carbonate apatite and an organic phase such as collagen. Synthetic carbonate apatite ceramics are considered as promising alloplastic materials for bone substitute. Chitin is the organic matrix of the hard parts of exoskeleton of insect, crustacean and present in a small amounts in mushrooms. It is an insoluble, similar to cellulose and composed of N-acetylglucosamine unit. Partial deacetylation from chitin result in the formation of chitosan. Chitin’s properties as a flexible and strong material make it favourable as surgical thread. It has novel properties such as biocompatibility, biodegradability, anti bacterial, wound healing activity, tissue regeneration and hemostatic activitities. The composit from carbonate apatite and chitosan may have a great impact on human health care system as bioresorbable bone substitute. The aim of the study was to evaluate the newly bone formation on the bone healing of defect tibia treated with carbonate apatite-chitosan bone substitute. Eighteen Sprague Dawley rats, male, 3 months, weighing 250-300g used in this study. Bilateral defect were created in each tibia rat. The defects were filled with carbonate-apatite chitosan bone substitute. The rats were sacrificed after respectively 1, 2 and 3 weeks. The result of this study showed that carbonate apatite-chitosan significantly increased a number of osteoblast (p<0.05). Carbonate apatite-chitosan group showed that matrix deposition faster than the other groups and have a good interface with the old bone. These data indicate that carbonate apatite-chitosan are potential candidate for bone substitute


Keywords


Carbonate apatite; chitosan; bone substitute

Full Text:

PDF


References

Kalfas IH. 2001. Principles of bone healing. Neu- rosurg Focus, 10(4): Article 1:1-4.

Murugan R, Ramakrishna S. 2003. Bioresorb- able composite bone paste using polysaccha- ride based nano hydroxyapatite. Biomaterials,25: 3829-3835.

Doi Y, Iwanaga H, Shibutani T, Moriwaki Y, Iwaya- ma Y. 1999. Osteoclastic responses to various calcium phosphates in cell culture. J. Biomed Mater Res, 47: 424-433.

Mangano C, Scarano A, Vittoria P, Giovanna L, Adriano P. 2007. Maxillary sinus augmentation with a porous synthetic hydroxyapatite and bovine-derived hydroxyapatite: A comparative clinical and histologic study. J Oral Maxillofac Implants, 22: 980-986.

Wollf KD, Swaid S, Nolte D, Bockmann RA, Holzle F, Mai CM. 2004. Degradable injectable bone cement in maxillofacial surgery:indication and clinical experience in 27 patients. J Cranio- Maxillofac Surg, 32: 71-79

Browaeys H, Bouvry P, De Bruyn HA. 2007. Liter- ature review on biomaterials in sinus augmen- tation procedures. Clin Implant Dent Relat Res, 9 (3): 166-77.

Hasegawa M, Doi Y, Uchida A. 2003. Cell-mediat- ed bioresorption of sintered carbonate patite in rabbits. J Bone Joint Surg (Br), 85-B: 142-7.

Ivanova TI, Kamenetskaya F, Kol’tsov, AB, Ugolk- ov VL. 2001. Crystal structure of calcium-defi- cient carbonated hydroxyapatite. thermal de- composition. Journal of Solid State Chemistry, 160: 340-349.

Matsuya S, Udoh K, Nakagawa M, Ishikawa K. 2005. Preparation of carbonate apatite mono- lith by treatment of the set gypsum containing calcite in trisodium phosphate solution. Archieves of Bioceramics Research, 5: 256-260.

Ana ID, Mudjosemedi M, Sofro ASM, Leeuwen- burgh SGC, Wolke JGJ, Ishikawa K, Jansen JA. 2007. Development of injectable carbonate apatite bone substitute based on phasetrans- formation of gypsum anf calcium hydroxyde: preliminart studies on factors influencing car- bonate apatite synthesis. Trend, Technology, and Innovation in Comprehensive Oral Health Care. 29th Asia Pasific Dental Conggres. Jakarta:147.

Khan TA, Peh KK, Ch’ng HS. 2000. Mechanical, bioadhesive strength and biological evaluations of chitosan films for wound dressing. J Pharm Pharmaceut Sci, 3(3): 303-311.

Han S. 2005. Topical formulations of water solu- ble chitin as a wound healing assistant- evalua- tion on open wounds using a rabbit ear model. Fibers and Polymers, 6 (3): 219-223.

Yubao ZL, Aiping Y, Xuelin P, Xuejiang W, Xiang Z. 2005. Preparation and in vitro investigation of chitosan/ nano-hydroxyapatite composite used as bone substitute materials. Journal of Materials Sciences: Material in Medicine, 16:213-219.

Matsuura A, Kubo T, Doi K, Hayashi K, Morita K, Yokota R, Hayashi H, Hirata I, Okazaki M, Akaga- wa Y. 2009. Bone formation ability of carbonate apatite-collagen scaffold with different carbon- ate contents. Dental Material Journal, 28(2):

-242

Chunmeng S, Ying Z, Xinze R, Meng W, Yong- ping S, Tianmin C. 2006. Therapeutic potential of chitosan and its derivatives in regenerative medicine. Journal of Surgical Research, 133: 185-192.

Hejazi R, Amiji M. 2003. Chitosan-based gastro- intestinal delivery system. J Control Release, 89: 151.

Khor E, Lim LY. 2003. Implantable applications of chitin and chitosan, Biomaterials, 24: 2339.



DOI: https://doi.org/10.22146/theindjdentres.10065

Article Metrics

Abstract views : 1299 | views : 1277

Refbacks

  • There are currently no refbacks.






  
   

 

 

 

website statistics

 

View My Stats