* Corresponding Author
Augmentation of Demineralized Bone Matrix Post-Tooth Extraction Increases The Density of Gingival Collagen Fiber of Rabbit Mandible
https://doi.org/10.22146/theindjdentres.10174 Regina TC Tandelilin(1*)
(1) Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
tissues defect after tooth extraction. The aim of this study was to determine the density of gingival collagen fiber on wound healing after the augmentation of DBM following the extraction of incisivus tooth. In this study, 36 male rabbits aged 2.5-3 months weighing 900-1,100 grams were randomly divided into two groups: control and treated rabbits. The incisivus teeth of mandibles of treated rabbits were extracted and augmented with the allograft DBM powder. The gingival was sutured with non-absorbable sutures. The same procedures were employed to the control group, except that these rabbits were augmented with DBM powder. Subsequently, the rabbits were sacrificed on days 1, 3, 5, 7, 10, and 14 after surgery, and each observation was represented by three rabbits in the sample. The gingival (ca. 0.5-1cm) was cut and fixed immediately in 10% paraformaldehide. The staining was done using van Geison. In the treated rabbits, the density of gingival collagen fiber significantly increases in all observation days except on first day, indicating that the allograft powder of DBM successfully accelerates the wound healing process.
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Mercier P. 1997. Resorption patterns of the residual ridge. In Block MS, Kent JN, editors Endosseus Implants for Maxillofacial
Reconstruction. W.B. Saunders Co Phil., 10-16.
Zoldos J, Kent JN. 1995. Healing of Endosseous Implants for Maxillofacial Reconstruction. WB Saunders Co. Phil.
Delloye, CH, 1999. The use of freeze-dried mineralized and demineralized bone. In Phillips GO, Strong DM, R von Versen. Nather A, editors Advances in Tissue Banking. World Scientific
Publishing Co. Pte Ltd Printed Singapore, Uto-Print, Vol 3.
Marx RE, Snyder RM, Kline SN. 1979. Cellular survival of human marrow during replacement of marrow-cancellous bone grafts. J Oral Surgery, 37.
William H Petri III, 1993. Theree M, Wilson. Clinical evaluation of antibiotic supplemented bone allograft. J Oral Maxillofacial Surgeon, 51: 982-5.
Reddi AH, Wientroub, Muthukumaran N. 1987. Biologic principles of bone induction. Orthopedic of North America, 18: 207-12.
Wang EA. 1993. Bone morphogenetic proteins (BMPs): Therapeutic potential in healing bone defects. Trends in Biotechnology, 11: 379-83.
Tandelilin RTC, Sofro ASM, Santosa AS, Soesatyo MHNE, Asmara W. 2006. The density of collagen fiber in alveolus bone of rabbit after augmentation with powder demineralized bone matrix post incisivus extraction. Dent. J, 39 (2): 43-7.
Tandelilin RTC. 2006. The effectivenes of Demineralized Bone Matrix augmentation on rabbit mandibular osteoblast density after incisor extraction. Indonesian J Dent. Special ed, 13: 297-302.
Wozney JM. 2002. Overview of bone morphogenetic proteins. Spine., 27 (165): 52-8.
Brunsvold A, Mellonig JT. 2000. Bone graft and periodontal regeneration. J Periodontology I:80-91.
Rubin E, Farber JL. 1995. Essential Pathology, 2nd ed. JB Lippincot Co. Philapdelphia.
Peterson LJ, Ellis III, Hupp JR, Tucker MR. 1997. Cotemporary Oral and Maxillofacial Surgery, 3rd ed. Mosby, St Louis.
Bennett NT, Schultz GS. 1993. Growth factors and wound healing: Biochemichal properties of growth factors their receptors. Am J Sur, 165: 728-37.
Cotran R, Kumar V, Collins T. 1999. Tissue Repair: Cellular Growth, Fibrosis, and wound healing. In Robbins Pathologic Basic of Disease. 8th ed, Philadelphia, WB. Sounders Co, 89-112.
Jungueira LC, Carneiro J. 1995. Histologi Dasar. Alih bahasa oleh Tambayong J. Edisi 3. Jakarta: Penerbit Buku Kedokteran EGC, 89-115, 370-81.
Prockop DJ, Kiviriko KI. 1995. Collagen: Molecular biology, disease, and potentials for therapy. Annu Rev Biochem, 64: 403-34.
Avery JK. 1994. Oral development and Histology. Second edition. Thieme Med Pub, Inc. New York.
Fawcett DW. 1994. A Textbook of Hystology, 12th ed. Chapman & Hall, New York, 71-90.
Cate T. 1994. Oral Histology, Development Structure and Function, 3rd ed. The CV Mosby Co. St Louis-Baltimore, Toronto, 89-245.
Phillips C, Wenstrup RJ. Biosynthetic and Genetic Disorders of Collagen. 1992. In Cohen IK, Dielgeman RF, Lindblad WJ, eds. Wound Healing – Biochemichal and Clinical Aspects: WB. Saunders Company.
Sodek J. 1977. A Comparison of the rates of synthesis and turnover of collagen and noncollagen protein in adult rat periodontal tissues and skin using a microassay. Arch Oral Biol, 22:
-65.
Marzoeki D. 1993. Ilmu Bedah-Luka dan Perawatannya. University Press- Surabaya, 4
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. 2000. Molecular Biology of The Cell, 3rd ed. Garland Publishing, New York, 403.
Yamaguchi Y, Yoshikawa K. 2001. Coetaneous wound healing: an update. J Dermatol, 28: 521-34.
Lajarva H, Salo T, Haapasaimi K, Kramer RH, Heino J. 1993. Expression of integrins and basement membrane components by wound keratinocytes. J Clin Invest, 92: 1425-35.
Hakkinen, L, Uitto, VJ, Larjava, H. 2000. Cell biology of gingival wound healing. Periodontology 2000, 24:127-52.
Fonseca RJ, Walker RV. 1991. Oral and Maxillofacial Trauma. WB Saunders Co. Philadelphia, 1: 13-31.
Eckes B, Zigrino P, Kessler D. 2000. Fibroblastmatrix interactions in wound healing and fibrosis. Matrix Biol, 19: 325.
Geiger B, Bershadsky A, Pankov R, Yamada KM. 2001. Transmembrane crosstalk between the extracellular matrix-cytoskeleton crosstalk. Nat Rev Cell Biol, 2: 793.
Graves DT, Concran DL. 1990. Mesenchymal cell growth factors. Crit Rev Oral Biol Med, 1: 17-36.
Deuel TF, Kawahara RS, Mustoe TA, Pierce AF. 1991. Growth factors ang wound healing: platelet-derived growth factor as a model cytokine. Ann Rev Med, 42: 567.
Heldin CH, Westermark B. 1999. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev, 79: 1283.
Blatti SP, Foster DN, Ranganatan G, Moses HL, Getz MJ. 1998. Induction of fibronectin gene transcription and mRNA is a primary response to growth factor stimulation of AKR-2B cells. Proc Natl Acad Sci USA, 85: 919.
Bauer EA, Cooper TW, Huang JS, Altman J, Deuel TF. 1993. Stimulation of in vitro human skin collagenase expression by platelet- derived growth factor B chain during wound repair in
humans. J Clin Invest, 91: 2065.
Canalis E, Mc Carty T, Centrella M. 1988. Effects of basic fibroblast growth factor on bone formation in vitro. J. Clin Invest, 81: 1572-7.
Woo HM, Kim MS, Kweon OK, Kim DY, Nam TC, Kim JH. Effects of amniotic membrane on epithelial wound healing and stromal remodelling after excimer keractectomy in rabbit cornea. Br. J. Opthalmol. 2001, 85: 345-9.
Reibman J, Meixler S, Lee TC. 1991. Transforming Growth Factor beta 1, a potent chemo-attractant for human neutrophils, by passes classic signaltransduction pathways. Proc Natl Acad Sci US, 88: 6805.
Roberts AB, Spron MB, Assoian RK, Smith J, Roche NS, Wakefield LM, Heine UI, Liotta LA, Kehrl JH, Faucci AS. 1986. Transforming Growth Factorbeta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc Natl Acad Sci US., 83 :4167-71.
Beck LS, Deguzman. 1991. Lee Wp, Xu Y, Mc Fatridge LA, Amento EP. TGF-beta 1 accelerates wound healing: reversal of steroid-impaired healing in rats and rabbits. Growth Factors, 5: 295.
Yanagihara K, Tsamraya M. 1992. Transforming growth factor beta 1 induces apoptotic cell death in cultured human gastric carcinoma cells. Cancer Res, 52: 40-2.
DOI: https://doi.org/10.22146/theindjdentres.10174
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