The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue’s Sprague dawley Rats

Kwartarini Murdiastuti(1*), Suryono Suryono(2), Aini Moeljono(3), Mefi Priba Sari(4), Rani Gamawati(5)

(1) Department of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd  (group C), 5th  (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.


Visible Light Cure; radiation; epithelial thickness; neutrophil; macrophage

Full Text:



Alatas Z. 2007. Efek Kesehatan Pajanan Radiasi 1. Dosis Rendah. Cermin Dunia Kedokteran 154: 17 -23.

Alatas Z, Lusiyanti Y. 2003. Efek Kesehatan 2. Radiasi Non Pengion pada Manusia. Cermin Dunia Kedokteran 138 : 34 -40.

Anusavice KJ. 1996. 3. Ilmu Bahan Kedokteran Gigi. 10th Ed. EGC, Jakarta: 232-236.

Fedi PF, Vernino AR, Gray JL. 2004. 4. Silabus Periodonti. 4th Ed. EGC. Jakarta.

Newman MG, Takei HH, Klokkevold PR, Carranza 5. FA. 2006. Carranza’s Clinical Periodontology, 10t Ed. Saunders Co., Los Angeles.

Guyton A.C and Hall J.E. 1997. Buku Ajar Fisiologi 6. Kedokteran. 9th Ed. EGC, Jakarta: 543.

Hamilton IA and Blackwood HJJ. 1974. Cell Renewal of Oral Mucosal Epithelial of Rat. Journal Anatomi 117 (2): 313-27.

Harrison JW and Jurosky KA. 1991. Healing of Surgical Wounds in Oral Mucoperiosteal Tissues. Journal of Endodontics 17 (9): 425-435.

Junquiera L.C and Carneiro J. 2007. Histologi Dasar: Text and Atlas. 10th Ed. EGC. Jakarta: 236-8.

Wataha JC, Lockwood PE, Lewis JB, Rueggeber FA, Messer RLW. 2004 . Biological effects of blue light from dental cure units, Dental Materials Journal 20: 150-7.

Willey JM, Sherwod LM, and Woollverton CJ. 2009. Prescott’s Principles of Microbiology. Mc Graw Hill Higher Education, Boston: 174.

Voskanyan KS. 2009. UV and Visible Light- 12. Induced Mutation in Eschericia Coli,

contrib/karin/index.html . Downloaded July 7th, 2010.

Wiseman H and Halliwell B. 1996. Damage to 13. DNA by Reactive Oxygen and Nitrogen Species: Role in Inflamatory Disease and Progression to Cancer, Biochem Journal 313: 17-29.Underwood JCE. 2000. 14. General and Systematic Pathology. 3rd Ed. Churchill Livingstone. London: 202-14.

Lawler W, Ahmed A, Hume WJ. 1992. 15. Buku Pintar Patologi untuk Kedokteran Gigi. EGC. Jakarta: 9-11.

Kumar V, Ramzi SC, Stanley LR. 1997. 16.

Basic Pathology. W.B. Saunders Company. Philadelphia: 26-40.

Roeslan BO. 2002. 17. Imunologi Oral. Faculty of Dentistry Universitas Indonesia. Jakarta: 5, 38.

Midwood KS, Williams LV, and Schwarzbauer 18. JE. 2004. Tissue Repair and te Dynamics of the Extracellular Matrix. The International Journal of Biochemistry and Cell Biology 36 (6):1031-7.


Article Metrics

Abstract views : 1413 | views : 1126


  • There are currently no refbacks.





website statistics


View My Stats