Potensi Bunga Telang (Clitorea ternatea L.) sebagai Pewarna Bakteri Sederhana Berbasis Bahan Alam Ramah Lingkungan

https://doi.org/10.22146/ijl.v0i3.82305

Atik Kurniawati(1*), Tanto Hariyanto(2), Hupitoyo Hupitoyo(3)

(1) Poltekkes Kemenkes Malang
(2) Poltekkes Kemenkes Malang
(3) Poltekkes Kemenkes Malang
(*) Corresponding Author

Abstract


Pewarnaan bakteri merupakan salah satu materi dari Praktikum Mikrobiologi yang harus ditempuh oleh mahasiswa di salah satu Program Studi Vokasi Kesehatan. Penggunaan pewarna bakteri sintetis berbahaya terhadap manusia dan lingkungan hidup karena bersifat mutagenik. Penelitian ini bertujuan untuk mengetahui potensi dari ekstrak kelopak bunga telang (Clitorea ternatea L.) sebagai alternatif pewarna bakteri sederhana berbasis bahan alam ramah lingkungan. Metode penelitian yang digunakan adalah eksperimen laboratorium dengan menggunakan analisis secara deskriptif. Hasil penelitian menunjukkan ekstrak kelopak bunga telang (C. ternatea L.) memiliki kemampuan untuk mewarnai sel bakteri Gram-positif Staphylococcus aureus dan bakteri Gram-negatif Escherichia coli dengan kontras yang dapat dilihat di mikroskop. Bila dilihat dari kualitas pewarnaan nya, preprat yang diwarnai dengan ekstrak kelopak bunga telang memberikan kualitas pewarnaan yang kurang baik bila dibandingkan pewarna bakteri seperti safranin dan kristal violet. Kesimpulan dalam penelitian ini adalah ekstrak kelopak bunga telang (C. ternatea L.) belum bisa dipergunakan secara langsung sebagai alternatif pewarna bakteri sederhana berbasis bahan alam ramah lingkungan karena membutuhkan penambahan co-pigment sebagai stabilisator dari antosianin.


Keywords


bakteri; pewarna; alami

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References

Aditiyarini, D., & Iswuryani, E. O. (2021). Effect of various factors on anthocyanins extraction from butterfly pea flower (Clitoria ternatea L.). Nova Biotechnologica et Chimica, 20(1). https://doi.org/10.36547/nbc.817

Afrianto, W. F., Tamnge, F., & Hasanah, L. N. (2020). Review: A relation between ethnobotany and bioprospecting of edible flower Butterfly Pea (Clitoria ternatea) in Indonesia. Asian Journal of Ethnobiology, 3(2). https://doi.org/10.13057/asianjethnobiol/y030202

Aziza, V., Ulimaz, T. A., Ustari, D., Suganda, T., Concibido, V., Irawan, B., & Karuniawan, A. (2021). Keragaman Fenotipik Bunga Telang Double Petal Asal Indonesia dan Thailand Berdasarkan Morfologi Bunga. Al-Kauniyah: Jurnal Biologi, 14(1). https://doi.org/10.15408/kauniyah.v14i1.15558

Charurungsipong, P., Tangduangdee, C., Amornraksa, S., Asavasanti, S., & Lin, J. (2020). Improvement of Anthocyanin Stability in Butterfly Pea Flower Extract by Co-pigmentation with Catechin. E3S Web of Conferences, 141. https://doi.org/10.1051/e3sconf/202014103008

Gupta, A., Sengupta, S., Nathan, A., Agarwal, A., Devadas, M., Madhumati, G., & Suneetha, V. (2014). Preparation of a microbial stain from the natural product kumkum for pharmaceutical applications. International Journal of Drug Development and Research, 6(4).

Jasphin, S., Raghavendra, A. P., Solomon, M. C., Amuthan, A., Singh, B. M. K., & Kairanna, N. V. (2023). Anthocyanins: A Hue For Histology - Systematic Review. Malaysian Journal of Science, 42(1). https://doi.org/10.22452/mjs.vol42no1.10

Jeyaraj, E. J., Lim, Y. Y., & Choo, W. S. (2020). Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. In Journal of Food Science and Technology. https://doi.org/10.1007/s13197-020-04745-3

Jiwintarum, Y., Rohmi, & Prayuda, I. D. P. M. (2016). Buah Naga (hylocereus polyrhizus) Sebagai Pewarna Alami untuk Pewarnaan Bakteri. Jurnal Kesehatan Prima, 10(2).

Li, H., Li, L., Chi, Y., Tian, Q., Zhou, T., Han, C., Zhu, Y., & Zhou, Y. (2020). Development of a standardized Gram stain procedure for bacteria and inflammatory cells using an automated staining instrument. MicrobiologyOpen, 9(9). https://doi.org/10.1002/mbo3.1099

Ludin, N. A., Al-Alwani, M. A. M., Mohamad, A. B., Kadhum, A. A. H., Hamid, N. H., Ibrahim, M. A., Teridi, M. A. M., Al-Hakeem, T. M. A., Mukhlus, A., & Sopian, K. (2018). Utilization of natural dyes from Zingiber officinale leaves and Clitoria ternatea flowers to prepare new photosensitisers for dye-sensitised solar cells. International Journal of Electrochemical Science, 13(8). https://doi.org/10.20964/2018.08.04

Moyes, R. B., Reynolds, J., & Breakwell, D. P. (2009). Preliminary staining of bacteria: Simple stains. In Current Protocols in Microbiology (Issue suppl. 15). https://doi.org/10.1002/9780471729259.mca03es15

Nunki, N., Titik Mutiarawati, D., & Prayekti, E. (2020). Purple Sweet Potato (Ipomoea batatas L.) Peels Extract as an Alternative Dye For Bacteria Gram Staining. Indonesian Journal of Medical Laboratory Science and Technology, 2(2). https://doi.org/10.33086/ijmlst.v2i2.1655

Purba, E. C. (2020). Kembang telang (Clitoria ternatea L.): Pemanfaatan dan Bioaktivitas. EduMatSains, 4(2).

Risandiansyah, R., Arniyati, A., Nurita, N. I., & Gionika, N. H. (2020). The Use of Food Coloring Dyes in Bacterial Staining. The Journal of Experimental Life Science (JELS), 10(2). https://doi.org/https://doi.org/10.21776/ub.jels.2020.010.02.10

Sanderson, J. (2020). Fundamentals of Microscopy. Current Protocols in Mouse Biology, 10(2). https://doi.org/10.1002/cpmo.76

Triol, C. B., Dionela, A. T. V, Ecube, A. I. D., Joy, C., Mediodia, A., Science, P., Visayas, W., Bito-on, B., & City, I. (2020). The use of Clitoria ternatea ( blue ternate ) ethanolic extract as a potential stain for bacteria. Department of Science and Technologi, Philippines, 3(1).

Vollmer, W., Blanot, D., & De Pedro, M. A. (2008). Peptidoglycan structure and architecture. In FEMS Microbiology Reviews (Vol. 32, Issue 2). https://doi.org/10.1111/j.1574-6976.2007.00094.x

Wanger, A., Chavez, V., Huang, R. S. P., Wahed, A., Actor, J. K., & Dasgupta, A. (2017). Biochemical Tests and Staining Techniques for Microbial Identification. In Microbiology and Molecular Diagnosis in Pathology (pp. 61–73). Elsevier. https://doi.org/10.1016/b978-0-12-805351-5.00005-3



DOI: https://doi.org/10.22146/ijl.v0i3.82305

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