Renoprotective Effect Of Ekstrak Etanol Goat Weed (Ageratum Conyzoides L) Against Paraquat- Induced Wistar Rats

haris setiawan; Apriyanti Apriyanti; Dita Rohmantin; Lubna Basalamah; Intan Faya Nurazizah; Jelia Enggal Listina

doi:10.22146/ijvs.v4i2.106862

Renoprotective Effect Of Ekstrak Etanol Goat Weed (Ageratum Conyzoides L) Against Paraquat- Induced Wistar Rats

https://doi.org/10.22146/ijvs.v4i2.106862

haris setiawan⁽¹⁾, Apriyanti Apriyanti⁽²⁾, Dita Rohmantin⁽³⁾, Lubna Basalamah⁽⁴⁾, Intan Faya Nurazizah⁽⁵⁾, Jelia Enggal Listina^(6*)

(1) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(2) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(3) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(4) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(5) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(6) Program Studi Biologi, Fakultas Sains dan Teknologi Terapan, Universitas Ahmad Dahlan
(*) Corresponding Author

Abstract

Pesticide poisoning is a public health problem in developing countries, with 300,000 deaths in Asia-Pacific. Paraquat is a gramoxone pesticide active ingredient that has long been used and can form Reactive Oxygen Species (ROS) that damage organs such as kidneys and liver. Goat weed plants contain flavonoids, alkaloids, essential oils, phenols, and coumarins, which have antioxidant effects. This study aims to determine the impact of goat weed plant extract (Ageratum conyzoides L.) on changes in rat kidney histology exposed to paraquat dichloride. The study involved 24 male Wistar rats with four treatments: C (distilled water), CN (paraquat), P1 (paraquat and goat weed extract 200 mg/kg BW), and P2 (paraquat and goat weed extract 400 mg/kg BW). The extracts and paraquat exposure were administered on days 1 to 7, with extract administration at one-hour intervals after paraquat exposure. On the 8th day, rat blood samples were taken for MDA and urea testing, and kidneys were taken for preparation using the paraffin method and hematoxylin-eosin staining. The main parameters analyzed included kidney index ratio, kidney weight, glomerular area, inflammatory area, necrosis cells, MDA, and urea levels. Analysis was performed using the ANOVA test and Duncan's further test (p<0.05). The results showed significant improvements in liver index ratio, liver weight, hepatocyte cell area, inflammation area, necrosis hepatocyte cells, MDA, and urea in the 400 mg/kg BW dose treatment (P<0.05). The conclusion shows that a dose of 400 mg/kg BW of goat weed ethanol extract can protect the kidneys and maintain MDA and urea levels of rats exposed to paraquat dichloride.

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DOI: https://doi.org/10.22146/ijvs.v4i2.106862