Detection of Bovine Herpes Virus –I Infection Causes Infectious Bovine Rhinotracheitis by Serological And Molecular in Imported Cattle

Abstract

Infectious Bovine Rhinotracheitis (IBR) is a disease in cattle caused by Bovine Herpesvirus 1 (BHV-1). IBR disease in cattle is spread all over the world, is very infectious, causes respiratory, reproductive, and nervous disorders. This disease is also economically detrimental in international trade. Cattle traffic from abroad is still quite high with a prevalence rate in Australia 15-96%. Subclinical infections often occur and also cause latent infections, so that antigen and antibody detection is needed to determine the health status of animals. This study aims to detect BHV-1 antibodies and antigens in imported cattle. Samples were taken from 25 imported cattle that entered through the Tanjung Intan port with two collections. The first and second collections were carried out at an interval of 1 week. The samples taken included serum samples and nasal swabs from cows showing clinical symptoms of IBR. The samples were then tested with an antibody Enzyme-Linked Immunosorbent Assay (ELISA) and real-time polymerase chain reaction (PCR). The test results showed that 64% were seropositive in the antibody ELISA test, while in the Real Time PCR test, 72% were positive. Both tests showed seropositive samples, 62.5% detected BHV-1 antigen and 37.5% did not detect BHV-1 antigen. Samples with seronegative results, 88% detected BHV-1 antigen. Conclusion. from this study, 40% of samples detected BHV-1 based on serology and real time PCR