Antibacterial activities of honey, Papaya leaf, basil leaf, and Temu ireng Extract on the growth of Salmonella typhimurium

Ghias Ghifari Alhadz; Agnesia Endang Tri Hastuti Wahyuni

doi:10.22146/ijvs.v2i2.83329

Antibacterial activities of honey, Papaya leaf, basil leaf, and Temu ireng Extract on the growth of Salmonella typhimurium

https://doi.org/10.22146/ijvs.v2i2.83329

Ghias Ghifari Alhadz⁽¹⁾, Agnesia Endang Tri Hastuti Wahyuni^(2*)

(1) Undergraduate Student of Faculty of Veterinary Medicine, UGM
(2) Gadjah Mada University
(*) Corresponding Author

Abstract

Indonesia is the second major nation in a term of its biodiversity after Brazil. Indonesia’s rich biodiversity makes Indonesian herbs a prospect of developing herbal medicines for the benefit of health and industrial products. Honey, papaya leaves, basil leaves, and temu ireng are natural and herbal that heve potential as an antibacterial. Salmonella typhimurium is a paratyphoid-causing bacteria that can harm the poultry industry. Paratyphoid is a disease that can cause weight loss and production in chickens. This study aimed to determine the antibacterial activity of honey, papaya leaves, basil leaves, and temu ireng to the growth of S. typhimurium.Reidentification of S. typhimurium was carried out to confirm the colony morphology, cell morphology, and biochemical characteristics. Bacteria were then cultured in Brain Heart Infusion and incubated at 37oC for 24 hours. The bacterial culture was centrifuged at a speed of 5000 rpm for 10 minutes. The precipitate formed is resuspended with sterile Phosphate Buffer Saline. The bacterial suspension is made with a concentration of 1.5 x 108 CFU/mL. Antibacterial activity of honey (100%), ethanol extract (100%) and aquadest extract (33,3%) from papaya leaves, basil leaves, and temu ireng against S. typhimurium were carried out by modified Kirby-Bauer disk diffusion method. The results of this study show that Kupang honey, Sumba honey, ethanol extract (100%) and aquadest extract (33,3%) from papaya leaves, basil leaves, and temu ireng have low antibacterial activity (<6mm). Whereas Gunung Kidul Lanceng honey(7.37mm), Lombok black honey (6.74 mm), Lombok white honey (7.74mm) and commercial honey (7.29) have moderate antibacterial activity.

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DOI: https://doi.org/10.22146/ijvs.v2i2.83329