Antibacterial Activities of Papaya Leaves (Carica papaya L.), Basil Leaves (Ocimum sanctum L.), Temu Hitam Rhizomes  (Curcuma aeroginosa Roxb.), and Honey Against Escherichia coli

Farah Nadila Sari; Agnesia Endang Tri Hastuti Wahyuni

doi:10.22146/ijvs.v3i2.83330

Antibacterial Activities of Papaya Leaves (Carica papaya L.), Basil Leaves (Ocimum sanctum L.), Temu Hitam Rhizomes (Curcuma aeroginosa Roxb.), and Honey Against Escherichia coli

https://doi.org/10.22146/ijvs.v3i2.83330

Farah Nadila Sari⁽¹⁾, Agnesia Endang Tri Hastuti Wahyuni^(2*)

(1) Undergraduate Student of Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract

Herbs extract such as papaya leaves, basil leaves, Curcuma aeroginosa and honey contain secondary metabolites which have the antibacterial ability to treat various types of health problems. The purpose of this research is to examine the antibacterial activity of papaya leaves, basil leaves and temu hitam rhizome extracts and honey against E. coli. We assume that the presence of antibacterial activity of natural ingredients can potentially be used as a substitute for AGP. Re-identification of E. coli isolates to ensure that the isolates were pure E. coli. After re-identification, E. coli was cultured on Brain Heart Infusion (BHI) and incubated 37oC for 18-24 hours. This research used 100% ethanol and 33.3% aquades of herbal extracts and 100% concentration of honey. E. coli culture was centrifuged at 5000rpm. The suspension of E. coli was spread on Mueller Hinton Agar (MHA), respectively, at the density of 1.5x108 CFU/mL. Kirby-Bauer diffusion disc method was performed to examine the antibacterial activities of herbs extracts and honey against E. coli. The results show a moderate inhibition zone (6-11 mm) in commercial honey and a low inhibition zone (less than 6 mm) in papaya leaves, basil leaves, and rhizomes of temu hitam.

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References

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DOI: https://doi.org/10.22146/ijvs.v3i2.83330