THE EFFECT OF THE NUMBER OF VISITORS ON TESTOSTERONE LEVELS IN THE FECES OF HORSES (equus caballus)  AT MINI ZOO JOGJA EXOTARIUM

Claude Mona Airin

doi:10.22146/ijvs.v4i1.90905

THE EFFECT OF THE NUMBER OF VISITORS ON TESTOSTERONE LEVELS IN THE FECES OF HORSES (equus caballus) AT MINI ZOO JOGJA EXOTARIUM

https://doi.org/10.22146/ijvs.v4i1.90905

Claude Mona Airin^(1*)

(1) Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Agro-tourism is a type of tourism that is currently in demand by tourists. One of the popular agro-tourism concepts is the one that carries the concept of a combination of animal farming and agriculture. This concept can be found at the Mini Zoo Jogja Exotarium. Mini Zoo Jogja Exotarium offers facilities for visitors to interact with various kinds of animals, including horses. This study aimed to examine the effect of the number of visitors at Mini Zoo Jogja Exotarium on testosterone levels in horse feces samples.The fecal samples that were being used were non-invasively collected for 12 days. Horse fecal samples were extracted using 80% methanol and then analyzed

quantitatively using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The results showed that the lowest horse testosterone level was 52.52 ng/gr dry feces when the number of visitors reached 199 people, while the highest testosterone level was 168.43 ng/gr dry feces when there were 26 visitors. The statistical analysis results show that both variables have -0.298 as a Correlation Coefficient. Based on the two analysis results, it can be concluded that the number of visitors has a weak and negative correlation with the metabolit testosterone horse.

Keywords: Horse, non-invasive, testosterone, feces, ELISA

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DOI: https://doi.org/10.22146/ijvs.v4i1.90905