Isolation of Bacteria Producing Enzyme Collagenase From Waste of Pufferfish (Arothon reticularis) Skin



Raden Lukas Martindro Satrio Ari Wibowo(1*), Nanung Agus Fitriyanto(2), Ambar Pertiwiningrum(3), Zaenal Bachruddin(4)

(1) Department of Leather Tanning Technologies. Polytechnic of ATK
(2) Department of Animal Product, Faculty of Animal Science, Universitas Gadjah Mada
(3) Department of Animal Product, Faculty of Animal Science, Universitas Gadjah Mada
(4) Department Nutrition and Animal Feed. Animal Science, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Puffer fish is one of the waste fisheries catch. It is not only can be a waste of fisheries that is difficult to degrade, but also can be used as a source of collagenolytic bacteria.The objective of this research was to obtain bacterial isolates from the waste of puffer fish skin (Arothon reticularis) as one of the sources of collagenases. Sample from puffer fish skin waste was inoculated in enrichment media and colonies producing clear zone in skim milk agar were selected and identified as B. cereus BRAW_KM. Medium optimization to grow the selected collagenase producing bacterial strain was checked with various parameters such as temperature (at 31 and 33oC), pH (8 to 9), substrate concentration (15 g/L), osmotic pressure (4%), inoculum concentration (8%), and agitation speed (100 to 120 rpm). The bacteria produced extracellularly collagenase enzymes in enrichment media and its collagenase activity was 1,029 U/mg.


Keywords


Bacteria; Collagenase; Isolation; Puffer fish skin; Waste

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