The Quality of Chilled Fat Tail Sheep Ram’s Semen with Antioxidant Addition, Vitamin C and Vitamin E In Citrate Egg Yolk Extender



labib Abdillah(1), Sigit Bintara(2*), Dyah Maharani(3), I Gede Suparta Budisatria(4)

(1) departmen of animal breeding and reproduction faculty of animal science ugm
(2) departmen of animal breeding and reproduction faculty of animal science ugm
(3) departmen of animal breeding and reproduction faculty of animal science ugm
(4) departmen of animal breeding and reproduction faculty of animal science ugm
(*) Corresponding Author

Abstract


The study was conducted to compare the effect of antioxidant supplementation in citrate egg yolk (CEY) extender on quality of chilled fat tail sheep (FTS) ram semen under field condition. This study used Citrat egg yolk (CEY) extender with the addition of antioxidant Vitamin C (0.5 gr/100 ml) and Vitamin E (0.4 gr/100 ml).  Semen was collected from FTS ram using artificial vagina. Only semen has motility 80% and abnormality under 20% were used for frozen semen process. Collected semen was divided into three tubes and extended with CEY (PO), CEY+Vitamin C (P1), CEY+Vitamin E (P2). The extended semen samples were chilled to 4°C, and observed four hours after stored in refrigerator. The result indicated PO had a greater quality than other treatment (P<0.05) on the motility, abnormality and viability of chilled ram semen, but no significant different in membrane integrity. In conclusion, Citrat egg yolk added with antioxidant Vitamin C and Vitamin E has not provided a protective effect in early hours of storage. Moreover, the addition of antioxidants could a slightly decreased the semen quality after dilution process.


Keywords


Citrate egg yolk; Antioxidant; DEG; Chilled semen

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