Study on Vaginal Epithelial Cells in Ongole Grade Cattle Suspected Reach Puberty

Abstract

Puberty is characterized by the time when cattle first estrus. Estrus in cattle can be seen through the cytology of the vaginal epithel (Vaginal smear). The study aimed to assess the vaginal wall cytology of Ongole Grade cattle predicted in the onset of puberty. Ongole Grade cattles were reared by a group of farmers which located in six sub-district: Mirit, Lembu Purwo, Ambal, Puring, Petanahan, and Klirong in Kebumen district, Central Java Province. The estimation of puberty was using 3 nonlinear mathematical models consisted of Gompertz (6.3 months, n = 4), Bertalanffy (7.2 months, n = 4), and Logistics (10.9 months, n = 7). Vaginal smear was done by smear wetted cotton bud using aquadest on Ongole Grade cattle vaginal wall, then it was applied to glass object. The glass objects were immersed in alcohol (70%) for 5 to 7 minutes, then it were immersed in liquid giemsa stain (5%) for 45 minutes. The glass objects were dried, then it were observed using a microscope with magnification 40 times. The vaginal smear samples were taken 8 times, and done every 3 days. The results showed that there were only parabasal and intermediates cell in all ages of cattle observed. This indicates that all the animals observed have not entered the age of puberty.