Optimization of Protein Isolation Technique on Pig Hair

Abstract

In the earlier research, the technique of protein isolation on pig hair took some weeks. The aim of the research was optimizing of the technique of the protein isolation on pig hair, so the protein could be used directly for detection of pig species. Samples were pig hairs and paintbrush. The protein isolation technique was optimized by cutting the hair in smaller pieces, using SDS in 2% and 3%, H₂SO₄  in 5% and 10%, and increasing sum of NH₄HCO₃ 50%. The concentration of protein product was analyzed by Kjeldahl method, while pig species was detected by pork Xema kit. The result showed the optimum technique used SDS 2%, H₂SO₄ 10%, and NH₄HCO₃ 50%. Protein of pig hair were 16.30% b/v of uncentrifuged protein, 18.02 % b/v centrifuged protein (top part), 17.16% b/v entrifuged protein (bottom part). Protein of painbrush: 14.18 % b/v uncentrifuged protein; 29.70 uncentrifuged protein (top part), 35.72% centrifuged protein (bottom part). The samples of pig hair and the brush were detected positively by pork Xema test.