Evaluation the Natural Proportion of X-Y Chromosome Bearing Sperm of West Java Local Ram Using Morfometric Methode

Abstract

Local Ram has a great contribution to supply of meat consumtion especially in West Java.  Reproductive biotechnology was used to ensure meat production such as sperm sexing technology. As a preliminary study of Sperm Sexing technology in Local ram, we should know the natural ratio of X-Y chromosome bearing sperm to develop the sperm sexing methode. The objective of this study were to evaluate the natural ratio of X-Y chromosome bearing sperm in Local Ram. The object of this research was semen from 30 Local Ram, and then was made differential preparat for counting the size of head sperm as much 200 sperm cell. Sperm whose head size is greater than the average is categorized as X chromosome bearing sperm (X sperm), and smaller than average is categorized as the Y chromosome bearing sperm (Y sperm). Analisys data consist of percentage of  X and Y chromosome bearing and then will avearage from 30 local Ram. The result of this study showed that of 30 Local Ram that were sampled had a sperm head length between 8.14 to 9.31 µm with an average of 8.66 µm; Sperm head width between 4.50 to 5.39 µm with an average of 4.93 µm; Sperm head area ranges from 22.97 - 59.30 µm² with an average of 36.82 µm². Based on the size of the sperm head, obtained the average proportion of sperm X and Y of 50.70% (X) and 49.30% (Y). Based on the research data, it is concluded that the proportion of X and Y chromosomes bearing sperm of West Java Local Ram approached the proportion of 50: 50, indicating that the birth of male and female lamb in Local Ram had equal opportunity value.