Micropropagation of Banana Plant (Musa paradisiaca) cv. Raja Bulu through Tissue Culture for Diversification of Food and Feed

Abstract

Banana cv. Raja Bulu is a perennial herbaceous monocot plant which is one of the main fruit crops in Indonesia. Banana plant residues can be used as feed for livestock. In order to produce faster and healthier plant, it is required an efficient micropropagation method. This research was aimed to optimize initiation, multiplication, and regeneration of shoot tip culture. Shoot tips from young suckers of 405100 cm height were used as e7plants. From the selected sucker a cube of tissue of about 152 cm³ containing the apical meristem was e7cised. This block  of tissue was dipped in 71% ethanol for 10 s, surface sterilized in a 1% sodium hypochlorite solution for 11 min, and rinsed three times in sterile water for 1 min. Then, a shoot tip of about   3 × 1 mm, consisting of the apical dome covered with several leaf primordial was aseptically dissected. The e7plant was placed on a multiplication5inducing culture medium which were Murashige and Skoog (MS) based medium supplemented with phytohormone BAP and NAA  were used for culture initiation and shoot multiplication. Of various treatment combinations, MS medium + 6 mg/L BAP with 0.2 mg/L NAA showed highest multiple shoots formation. For rhizogenesis, individual shoot was transferred to MS medium + 1 mg/L NAA and 10 mg/L activated charcoal.