Reduction of phytic acid and aflatoxin content to rice bran through fermentation by Rhizopusspp. combined with deproteinated chitin waste addition

Abstract

The research was conducted to evaluate the effect of deproteinated-chitin waste
(DCW) addition on rice bran fermented by Rhizopusspp.asan animal feed. Waste from deproteinated- chitin contain essential mineral as supported nutrient sources for inoculum growth. The experiment was designated into two treatments consisted of rice bran without fermentation/control (T0) and fermentation by Rhizopusspp.combined with addition of DCW (T1). Parameters measured were dry matter, ash, crude protein, crude fiber, ether extract and nitrogen free extract. Aflatoxin and phytic acid were also analyzed to evaluate antinutrient factors. Data were analyzed using least significant difference (LSD) to compare between treatment means. Results indicated that treatment affectedthe quality of rice bran. Phytic acid and crude fiber contents on rice bran treated by T1 decreased 35.1% and 38.4%,respectively, comparedwith control (T0). Aflatoxin also tended to decrease by treatment (T1) i.e. 89.4% less than control (T0). Ether extract of fermented rice bran increased from 10.5% to 16.9%, conversely nitrogen free extract reduced from 50.4% to 49.1%. Mineral content increased from 1.08% to 1.31% and 0.63% to 0.76%, respectively, for Ca and P contents, in which positively correlatedwith increasing ash. However, treatment did not affect crude protein content (17.6 vs.16.9%).It was concluded that combination treatment of DCW addition and fermentation using inoculum Rhizopusspp. improved rice bran quality.