The effect of methionine on glutathion production to eliminate aflatoxin b1 toxicity

Abstract

This trial was conducted to study the conversion of methionine on feed to glutathione (GSH) as an effort for elimination of Aflatoxin B1 (AFB1) toxicity. Twenty five male Lohmann broilers DOC were used in this trial, divided into 5 groups of treatment with 5 replications. Every DOC in each replication was kept on individual cage. Aflatoxin B1 used in this trial was pure AFB1 with the concentration of 1000µg/kg of feed, while the methionine concentrations in diet were 0.5
(P1), 0.75 (P2), 1.0 (P3), 1.25 (P4) and 1.5% (P5). The trial was conducted for 5 weeks, whereas at the 3rd and 5th week the blood sample were collected for GSH analysis. At the 5th week, chickens were slaughtered and the liver was taken for histology and morphology observation. Data observed were daily gain, feed intake and feed conversion. Collected data were analyzed by analysis of variance using completely randomized design (CRD) and continued with Duncan’s test if there was any differences between the treatments. The result showed that there was kinier correlation between supplementation of methionine with production of GSH as it was described on the equation, y = 0,135x2 – 0,313x + 5,046 (R2 = 0,94) for 21 days old broiler and y =1.763 Ln(x) + 7.111 (R2=0.98)for 35 days old broiler. On the broiler performance, methionine supplementation could reduce feed conversion. Methionine supplementation could prevent the liver necrosis, except for 1.5% methionine concentration. It was concluded that the use 0.75-1.25% of methionine on the broiler feed could increase the GSH production, so it could be used as a method for elimination of Aflatoxin B1 toxicity