The effects of single lactic acid bacteria probiotic supplementation on intestinal mucosa profile and immune response in broilers

Abstract

The aim of this study was to examine the effects of single lactic acid bacteria probiotic supplementation on intestinal mucosa profile and immune response in broiler chicks. In randomized design, sixty 7-days-old broiler chicks were assigned to 4 treatments with 3 replicates and 5 chicks in experimental unit with water and allow to feed ad libitum. The experimental treatments were added to basal (starter and finisher) diets (without added antibiotics, coccidiostats or growth promoter) as follow: T(0): control group (C) that received starter and finisher diets, T(1): C added daily Lactobacillus murinus for 21 days, T(2): C added daily Streptococcus thermophilus for 21 days and T(3): C added daily Pediococcus acidilactiti for 21 days. All chicks were given the commercial Avian influenza and Newcastle disease vaccines, the former was given intra muscularly and the latter was given ocularly. Result showed that additive on all single lactic acid bacteria (Lactobacillus murinus, Streptococcus thermophilus, and Pediococcus acidilactiti) significantly increased villi high of duodenum, jejunum, and ileum (P<0.05) as well as villi width of duodenum, jejunum, and ileum (P<0.05) compared with the control group. Additives all of the single lactic acid bacteria significantly (P<0.05) increased blood Avian influenza antibody titer compared with the control group. Result indicated that, supplementation of single lactic acid bacteria had positive effect on intestinal mucosa profile and blood antibody titers.