Methane Production and Rumen Fermentation Characteristics of Buffalo Ration Containing Sorghum Silage with Rumen Simulation Technique (RUSITEC) Methods

Abstract

This study was conducted to evaluate buffalo ration containing samurai 2 sorghum straw silage compared with buffalo ration containing pahat sorghum straw silage using rumen simulation technique (RUSITEC) method. RUSITEC Analysis was conducted for eleven days, consisted of ve days for adaptation period and six days another for observation period. Statistics design with 2 treatments and 3 replications was applied in this experiment. The fermentation of responses by time were presented descriptively. Averages of responses were analyzed using student t-test. Two treatments ration were: P1 (50% pahat sorghum straw silage + 50% concentrate) and P2 (50% samurai 2 sorghum straw silage + 50% concentrate). Variables measured were total gas, methane (CH4) production and ruminal fermentation products. Results showed that buffalo ration containing silage of Samurai 2 straw (P2) produced 27.76% lower methane production than buffalo ration containing silage of Pahat straw (P1) (P<0.01). The P2 treatment also produced the highest VFA 24.35% compared to P1, IVDMD (6.22%) and IVOMD (10.56%) (P<0.05). The pH value and total gas production were not signi cant different. The conclusion showed that buffalo ration containing silage of Samurai 2 straw produced the lowest methane concentration, the highest total VFA, IVDMD and IVOMD.