Characteristics of Polyunsaturated Fatty Acids and Nutrient Digestibility Feed Cattle of the Fermented Rumen Fluid by One and Two Stage in Vitro

Abstract

The objective of this research was to determine the effect of supplementation with polyunsaturated fatty acids (PUFA) protected in the feeding beef cattle based on the content of PUFA and the nutrients digestibility parameters by in vitro one-stage and two-stage. Fatty acid supplement derived from soya and lemuru sh oil. Soya groats and lemuru sh oil (SoyLem) both mixed with a ratio of 4: 1 are protected by an aldehyde of the formaldehyde concentration 37% as much as 2% of the dry matter. Fermentation was observed at one stage (48 hours) and two stage (96 hours) after incubation by in vitro. Rumen uid were taken from the Ongole crossbred cow stulated. Feed treatments including T1 = 30% elephant grass + 30% rice straw + 25% basal concentrate + 15% SoyLem unprotected, T2 = 30% elephant grass + 30% rice straw + 25% basal concentrate + 7.5% SoyLem unprotected + 7.5% SoyLem protected, and T3 = = 30% elephant grass + 30% rice straw + 25% basal concentrate + 15% SoyLem protected. The results of this study were the rumen fermentation in vitro showed palmitic C16:0), linoleat (C18:2), linolenat (C18:3), gama linolenat (C20:3) and arachidonat (C20:4) fatty acid content signi cantly different (P <0.05) at one stage of the incubation while gama linolenat (C20:3) dan arachidonat (C20:4) fatty acid content signi cantly different (P <0.05) at two stage of the incubation. Digestibility of dry matter, organic matter and crude protein were signi cantly different (P<0.05) in both the incubation one stage and two stages. The conclusion of research is a mixture of soya groats and lemuru sh oil (4: 1) protected aldehydes can be used as a supplement of up to 15% in cattle feed rich in fatty acids and without disturbing the digestibility by in vitro one-stage and two-stage.