Organic Acid and Inhibition of Complete Silage Ration on the Growth of Salmonella enteritidis

Abstract

Giving feed by silage technology is expected to replace the bene ts of antibiotics in poultry feed. The purpose of this study was to determine organic acids content and the ability of silage ration complete in Salmonella enteritidis inhibiting in vitro. Silage complete ration composed of some feedstuffs with the crude protein content of 21% and an additional 1 ml inoculum per 1 liter of water was added until 45% water content. Inoculum contained a total BAL 5 x 108 cfu/ml. An aerobic fermentation process was done by 1 kg of complete feed compacted into plastic and put into silos, stored according to treatment. Organic acids content were: lactic acid, propionic acid, butyric acid analyzed using HPLC with a wavelength of 215 nm. Completely randomized design was apllied in this experiment with 4 treatments stored (7, 14, 21, and 28 days) each repeated four times, the data was tested using SPSS, differences between treatments were tested by Duncant. The average results showed that lactic acid very signi cantly higher (P <0.01) on each day of observation: 7 days = 305.64 ppm; 14 days = 876.52 ppm; 21 days = 1666.79 ppm; 28 days = 4038.70 ppm. Propionic acid content on 7 and 14 days were not signi cantly differently: 164.1 ppm and 158.9 ppm, while on 21 days and 28 days storaged very signi cantly higher (P <0.01): 244.2 ppm and 325.9 ppm. However, butyric acid content below the detection limits. Inhibition activity analyzed using agar well diffusion method. Silage ration complete with 100% concentration was capable for forming a clear zone with the average area 7,61 mm against 106 cfu/ml of Salmonella enteritidis. In conclusion silage ration complete with 21% protein had a number of organic acids that could inhibit pathogenic bacteria so that poultry feed was safe and free from antibiotics.