The Fat Protective Effect of Fish Oil, Sunflower Seed Oil and Corn Oil on Fluid Rumen Fermentation Parameters

Abstract

This study was aimed to obtain oil and the exact saponi cation optimization to protect unsaturated fats, which does not interfere with the fermentation of rumen uid. The studied the protective effects of unsaturated fat as sheep feed supplement on fermentation parameters of sheep rumen uid in vitro. Three oil comparising lemuru sh oil (LFO), sun ower seed oil (SSO) and corn oil (CO) each made with a combination of saponi cation soap and capsulation. Manufacturing unsaturated fatty acids soap was protected by heating and stirring each oil and 20% NaOH solution (caustic soda), added with10% starch solution to form soft and elastic paste (gel). Ratio of oil volume ratio, 20% NaOH solution and starch was 1: 2: 1. Pasta was allowed to solidify within one night (12 hours), then sliced into thin slats with knife and soaked in saturated CaCl2 solution to harden the gel slats which was then crushed by squeezing to form small grains (crystals). Then dried (moisture content of 10 to 20%). Soap samples were taken for in vitro analysis to determine the parameters of the rumen fermentation conditions by observing parameters rumen uid pH, VFA, NH3, and rumen microbial protein. The results showed that the three oils were not signi cantly affecting (P>0.05) pH, microbial proteins, VFA, and NH3, except for signi cantly low digestibility of dry matter (DM) and organic matter (OM) (P<0.01) compared with no soap. Conclusively, protection of unsaturated fats did not interfere with rumen microbial fermentation and was safe from degradation in the rumen (shown with a normal pH and in vitro DM digestibility and very low OM).