Identification of Pure Breed Bali Cattle by Using Molecular Approach

Abstract

Bali cattle are well known as local beef cattle from Indonesia and recognized as one of beef cattle gene pools in South East Asia. The Bali cattle now are distributed almost throughout of Indonesia and most of them are in the status of either inbreeding or crossed breed. This recent research was intended to identify the pure breed of Bali cattle by using molecular marker approach. DNA Bali cattle samples collected from several locations in Indonesia were investigated. Amount of 24 DNA samples (23 males, 1 female) were used. Those 23 male DNAs consisted of male Bali cattle from NTB/West Nusa Tenggara (10), Riau (5), Bali (5), Kalimantan (2), and 1 male Brahman as breed control collected from Sembawa (Palembang) while one (1) DNA female was Bali cattle as sex control. An UTY gene was used in this study. All 24 DNA samples were ampli ed with UTY (F and R) primer for 35 cycles. Visualization of all PCR products on 1% agarose gel showed bands at 484 bp. as a right size for UTY gene, and none UTY fragment for female DNA sample (Bali cattle). Six UTY fragments were sequenced as representative of each region. The molecular analysis by ClustalW Alignment of the sequence results with reference of Genbank-NCBI showed that there was not found nucleotide different between sequenced samples to UTY reference, however there found 16 nucleotide different of sequenced samples to UTY gene reference. Similarity of UTY sequence was found 100% for sequence samples to UTY Bos Taurus and Bos indicus. This study concluded that UTY gen exists in all male Bali and male Brahman cattle. This early nding suggests that purity identi cation of Bali cattle needs more speci c genetic marker in the Y-chromosome.