Indonesian Research Institute for Animal Production, Indonesia

Abstract

The aim of this research is to determine the effect of sperm separation using albumin column with the modi cation of the column length and time of separation on sperm quality and the effectiveness of sperm separation. Semen was collected using an AV, evaluated and then separated using albumin column technique. The design of this research was factorial 2x2 with two treatments of length column: 2 ml (control) vs 3 ml (treatment) and two treatments of time separation (15 vs 30 minutes. The percentages of motility, live and dead and TAU of separated sperm were better in upper fraction compare to the lower fraction in both of the control group and the treatment group. The sperm motility in control group was 74.0 - 75.6% and 62.5 - 63.9% for upper fraction and lower fraction respectively. Compare to the treatment group were 70-70% dan 46 – 50% % for upper fraction and lower fraction respectively. Sperm concentration had a tendency to be reduced in the lower fraction and treatment group. The results form the control group showed that the ratio of X and Y sperm could be changed from 48%:52% (fresh semen) to 57%: 42.5% (separation time: 15 minutes) and 83.75%:16.25% (separation time: 30 minutes) for the upper fraction. For the lower fraction the ratio of X:Y were 30%:70% (15 minutes) and 26.25%:73.75% (30 minutes). In the other hand, form the treatment group of 30-minute time separation, showed that the ratio of X and Y were 85%:15% (upper fraction) and 21.70%:78.30% (lower fraction). From the results, it can be concluded that the technology of sperm separation column albumin is still effective to separate X and Y sperm both in sperm quality after separation and the sperm X and Y ratio achieved.