The Effect of Trehalose Level in Tris-Based Medium on Sperm Membrane Integrity of Boer Goat Semen after Cooling

Abstract

This study was conducted to evaluate the effect of trehalose level in tris-based medium on sperm membrane integrity of Boer goat semen after cooling. Semen was collected from 6 bucks using arti cial vagina. Fresh semen evaluated for colour, pH, volume, concentration, mass motility, individual motility, life sperm, sperm abnormality and sperm membrane integrity. Semen was diluted with tris-based medium suplemented with different level of trehalose (1.5; 2.5 and 3.5%) with the ratio of 1 semen : 9 diluter. Sampling was conducted as purposive sampling, i.e. semen used should has mass motility of 2+ and individual motility of 70%. Immediately after dilution semen was stored in 3-5oC and sperm membrane integrity percentage was observed at 0, 24 and 48 h. The obtained data were analyze with Analysis of Variance (ANOVA) and continued by Least Signi cant Different if there was signi cant or very signi cant difference between groups. The experiment was designed using completely random design (3 treatments and 10 replications). The results showed that the level of trehalose (1.5, 2.5 and 3.5% had very signi cant effect (P<0,01) on sperm membrane integrity percentage in 0 h of cooling (65.99; 64.67 and 57.46% respectively), 24 h of cooling (56.58; 55.89 and 48.80% respectively); 48 h of cooling (47.49; 48.75 and 39.38% respectively). It was concluded, that the trehalose levels for resulting in optimal sperm membrane integrity were 1.5%. It was suggested, that for resulting in optimal sperm membrane integrity after cooling of Boer goat semen in tris-based medium should be supplemented with 1.5% trehalose.