A New Technique to Detect Pig Hair by Immunochromatographic Rapid Test

Abstract

Pig hair can be used as brush to smear bread/cookies/other food or to clean devices for example a baking pan, so the food can be contaminated by haram stuff. As a brush, pig hair has no root of hair. If root of hair with some blood and skin are examined, DNA typing usually can be performed by moleculer biological technique. But, hair ends of the brush contain a lot of protein. Thus, the objective of the research is to develop a new technique to detect pig hair. The samples were pig hair ends without their root. The protein of the hair was biochemically extracted, and then protein was detected by immunochromatographic rapid test kit. Hair was rinsed by SDS 2% and PBS, and digested by H2SO4 10%. The protein content was estimated by Nynhydrin 5%, and was tested for pig species by Xema pork rapid test. The result showed that the protein could be collected, and the sample was detected positively as pig species.