Improvement quality of Bligon goat sperm trough separation by albumen

Abstract

This experiments were conducted to evaluate the influence of different concentration of albumen gradient in sperm separation on sperm quality of Bligon goat. Semen from 8 heads male Bligon goats aged around 2 years was collected by artificial vagina with frequency of once in four days. Sperm separation was conducted with different albumen gradient. Medium I: 5% upper fraction and 10% lower fraction, medium II: 10% upper fraction and 30% lower fraction, and medium III:15% upper fraction and 45% lower fraction. The results showed sperm separation with different albumen gradient was significantly difference (P<0.01) on motility, viability, and abnormality of sperm, respectively. Sperm motility before separation and after separation on medium I, II and III upper fraction was 80.5; 82.1; 83.5 and 85.2%, lower fraction was 80.5; 86.1; 87.2 and 88.3% respectively. Sperm viability before separation and after separation on medium I, II and III upper fraction was 90.4; 93.6; 93.1 and 93.6%, lower fraction was 90.4; 86.1; 87.2 and 88.3% respectively. Sperm abnormality before separation and after separation on medium I, II and III upper fraction was 13.1; 11.2; 9.2 and 7.1%, lower fraction was 13.1; 6.1; 5.3 and 4.2% respectively. The conclusion of this study was that there was albumen gradient may be used for improve sperm quality by increase motility and viability and decrease abnormality.