The early identification of twinning trait genes on Indonesian local beef cattle

Abstract

One of ways to increase local beef production is increasing beef cattle population with exploit genetic potency of twinning trait genes. This research was done as earliest identification to get basic data about twinning genes trait on PO cattle and Bali cattle as Indonesian local beef cattle. The research using five dams Bali cattle with nine her twinning calves and five dams with five her single calves that distributed on 10 farmers at Lombok island in West Nusa Tenggara province; also 10 dams PO cattle with 19 her twinning calves and five dams with five her single calves that distributed on 14 farmers at Hulu Sungai Tengah district in South Kalimantan province. DNA were isolated from the blood samples of every cattles were analyzed with PCR and sequencing to known genetic ban map and configuration specific of nucleate acids that cath up IGF-1 and IGF-2 genes as the twinning trait. The parameters were : electrophoresis fotos of genetic ban map, and configuration specific of nucleate acids that cath up IGF-1 and IGF-2 genes. Data were analyzed with descriptif. The results of this research showed that genetic ban map and configuration specific of nucleate acids that cath up IGF-1 and IGF-2 genes, at Bali cattle was not same as at PO cattle, also between cattles with twinning history and cattles with single history; but between dams and her calves were resembles, especially at cattles with twinning history. This study concluded that, the twinning trait leader on PO cattles and Bali cattles, could to be identified of their genetic with saw genetic ban map and configuration specific of nucleated acids that cath up IGF-1 and IGF-2 genes.